8pei: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8pei is ON HOLD  until Paper Publication
+==Crystal structure of the biphotochromic fluorescent protein SAASoti (C21N/V127T variant) in its green on-state==
+<StructureSection load='8pei' size='340' side='right'caption='[[8pei]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8pei]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stylocoeniella_armata Stylocoeniella armata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PEI FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5SQ:2-[2-[(1~{S})-1-AZANYL-2-(1~{H}-IMIDAZOL-4-YL)ETHYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXIDANYLIDENE-IMIDAZOL-1-YL]ETHANOIC+ACID'>5SQ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pei OCA], [https://pdbe.org/8pei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pei RCSB], [https://www.ebi.ac.uk/pdbsum/8pei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pei ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Wild-type SAASoti and its monomeric variant mSAASoti can undergo phototransformations, including reversible photoswitching of the green form to a nonfluorescent state and irreversible green-to-red photoconversion. In this study, we extend the photochemistry of mSAASoti variants to enable reversible photoswitching of the red form. This result is achieved by rational and site-saturated mutagenesis of the M163 and F177 residues. In the case of mSAASoti it is M163T substitution that leads to the fastest switching and the most photostable variant, and reversible photoswitching can be observed for both green and red forms when expressed in eukaryotic cells. We obtained a 13-fold increase in the switching efficiency with the maximum switching contrast of the green form and the appearance of comparable switching of the red form for the C21N/M163T mSAASoti variant. The crystal structure of the C21N mSAASoti in its green on-state was obtained for the first time at 3.0 A resolution, and it is in good agreement with previously calculated 3D-model. Dynamic network analysis reveals that efficient photoswitching occurs if motions of the 66H residue and phenyl fragment of chromophore are correlated and these moieties belong to the same community.
-Authors: Boyko, K.M., Varfolomeeva, L.A., Matyuta, I.O., Gavshina, A.V., Solovyev, I.D., Popov, V.O., Savitsky, A.P.
+The role of the correlated motion(s) of the chromophore in photoswitching of green and red forms of the photoconvertible fluorescent protein mSAASoti.,Gavshina AV, Solovyev ID, Khrenova MG, Boyko KM, Varfolomeeva LA, Minyaev ME, Popov VO, Savitsky AP Sci Rep. 2024 Apr 16;14(1):8754. doi: 10.1038/s41598-024-59364-1. PMID:38627478<ref>PMID:38627478</ref>
-Description: Crystal structure of the biphotochromic fluorescent protein SAASoti (C21N/V127T variant) in its green on-state
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Savitsky, A.P]]
+<div class="pdbe-citations 8pei" style="background-color:#fffaf0;"></div>
-[[Category: Gavshina, A.V]]
+== References ==
-[[Category: Varfolomeeva, L.A]]
+<references/>
-[[Category: Solovyev, I.D]]
+__TOC__
-[[Category: Popov, V.O]]
+</StructureSection>
-[[Category: Matyuta, I.O]]
+[[Category: Large Structures]]
-[[Category: Boyko, K.M]]
+[[Category: Stylocoeniella armata]]
+[[Category: Boyko KM]]
+[[Category: Gavshina AV]]
+[[Category: Matyuta IO]]
+[[Category: Popov VO]]
+[[Category: Savitsky AP]]
+[[Category: Solovyev ID]]
+[[Category: Varfolomeeva LA]]