8sod: Difference between revisions
New page: '''Unreleased structure''' The entry 8sod is ON HOLD Authors: Chen, C.-L., Tesmer, J.J.G. Description: Phosphoinositide phosphate 3 kinase gamma bound with ADP and two Gbetagamma subun... |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Phosphoinositide phosphate 3 kinase gamma bound with ADP and two Gbetagamma subunits in State 1== | ||
<StructureSection load='8sod' size='340' side='right'caption='[[8sod]], [[Resolution|resolution]] 3.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8sod]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SOD FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8sod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8sod OCA], [https://pdbe.org/8sod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8sod RCSB], [https://www.ebi.ac.uk/pdbsum/8sod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8sod ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/GBB1_BOVIN GBB1_BOVIN] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The conversion of phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 3,4,5-triphosphate by phosphoinositide 3-kinase gamma (PI3Kgamma) is critical for neutrophil chemotaxis and cancer metastasis. PI3Kgamma is activated by Gbetagamma heterodimers released from G protein-coupled receptors responding to extracellular signals. Here we determined cryo-electron microscopy structures of Sus scrofa PI3Kgamma-human Gbetagamma complexes in the presence of substrates/analogs, revealing two Gbetagamma binding sites: one on the p110gamma helical domain and another on the p101 C-terminal domain. Comparison with PI3Kgamma alone reveals conformational changes in the kinase domain upon Gbetagamma binding that are similar to Ras.GTP-induced changes. Assays of variants perturbing the Gbetagamma binding sites and interdomain contacts altered by Gbetagamma binding suggest that Gbetagamma recruits the enzyme to membranes and allosterically regulates activity via both sites. Studies of zebrafish neutrophil migration align with these findings, paving the way for in-depth investigation of Gbetagamma-mediated activation mechanisms in this enzyme family and drug development for PI3Kgamma. | |||
Molecular basis for Gbetagamma-mediated activation of phosphoinositide 3-kinase gamma.,Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG Nat Struct Mol Biol. 2024 Aug;31(8):1198-1207. doi: 10.1038/s41594-024-01265-y. , Epub 2024 Apr 2. PMID:38565696<ref>PMID:38565696</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8sod" style="background-color:#fffaf0;"></div> | ||
[[Category: Chen | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bos taurus]] | |||
[[Category: Large Structures]] | |||
[[Category: Sus scrofa]] | |||
[[Category: Chen C-L]] | |||
[[Category: Tesmer JJG]] | |||
Latest revision as of 08:29, 28 August 2024
Phosphoinositide phosphate 3 kinase gamma bound with ADP and two Gbetagamma subunits in State 1Phosphoinositide phosphate 3 kinase gamma bound with ADP and two Gbetagamma subunits in State 1
Structural highlights
FunctionGBB1_BOVIN Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Publication Abstract from PubMedThe conversion of phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 3,4,5-triphosphate by phosphoinositide 3-kinase gamma (PI3Kgamma) is critical for neutrophil chemotaxis and cancer metastasis. PI3Kgamma is activated by Gbetagamma heterodimers released from G protein-coupled receptors responding to extracellular signals. Here we determined cryo-electron microscopy structures of Sus scrofa PI3Kgamma-human Gbetagamma complexes in the presence of substrates/analogs, revealing two Gbetagamma binding sites: one on the p110gamma helical domain and another on the p101 C-terminal domain. Comparison with PI3Kgamma alone reveals conformational changes in the kinase domain upon Gbetagamma binding that are similar to Ras.GTP-induced changes. Assays of variants perturbing the Gbetagamma binding sites and interdomain contacts altered by Gbetagamma binding suggest that Gbetagamma recruits the enzyme to membranes and allosterically regulates activity via both sites. Studies of zebrafish neutrophil migration align with these findings, paving the way for in-depth investigation of Gbetagamma-mediated activation mechanisms in this enzyme family and drug development for PI3Kgamma. Molecular basis for Gbetagamma-mediated activation of phosphoinositide 3-kinase gamma.,Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG Nat Struct Mol Biol. 2024 Aug;31(8):1198-1207. doi: 10.1038/s41594-024-01265-y. , Epub 2024 Apr 2. PMID:38565696[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||