8sms: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 8sms is ON HOLD
+==Crosslinked Crystal Structure of Type II Fatty Acid Synthase, FabB, and cerulenin crosslinker-crypto Acyl Carrier Protein, AcpP==
+<StructureSection load='8sms' size='340' side='right'caption='[[8sms]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8sms]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atlantibacter_hermannii_NBRC_105704 Atlantibacter hermannii NBRC 105704] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SMS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G7U:N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-{2-[(2R)-2-hydroxy-4-oxododecanamido]ethyl}-beta-alaninamide'>G7U</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8sms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8sms OCA], [https://pdbe.org/8sms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8sms RCSB], [https://www.ebi.ac.uk/pdbsum/8sms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8sms ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FABB_ECOLI FABB_ECOLI] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Protein-reactive natural products such as the fungal metabolite cerulenin are recognized for their value as therapeutic candidates, due to their ability to selectively react with catalytic residues within a protein active site or a complex of protein domains. Here, we explore the development of fatty-acid and polyketide-synthase probes by synthetically modulating cerulenin's functional moieties. Using a mechanism-based approach, we reveal unique reactivity within cerulenin and adapt it for fluorescent labeling and crosslinking of fatty-acid and iterative type-I polyketide synthases. We also describe two new classes of silylcyanohydrin and silylhemiaminal masked crosslinking probes that serve as new tools for activity and structure studies of these biosynthetic pathways.
-Authors: Jiang, Z., Chen, A., Chen, J., Sekhon, A., Louie, G.V., Noel, J.P., La Clair, J.J., Burkart, M.D.
+Masked cerulenin enables a dual-site selective protein crosslink.,Jiang Z, Chen A, Chen J, Sekhon A, Louie GV, Noel JP, La Clair JJ, Burkart MD Chem Sci. 2023 Sep 8;14(39):10925-10933. doi: 10.1039/d3sc02864j. eCollection , 2023 Oct 11. PMID:37829009<ref>PMID:37829009</ref>
-Description: Crosslinked Crystal Structure of Type II Fatty Acid Synthase, FabB, and cerulenin crosslinker-crypto Acyl Carrier Protein, AcpP
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Chen, J]]
+<div class="pdbe-citations 8sms" style="background-color:#fffaf0;"></div>
-[[Category: Chen, A]]
+== References ==
-[[Category: La Clair, J.J]]
+<references/>
-[[Category: Noel, J.P]]
+__TOC__
-[[Category: Louie, G.V]]
+</StructureSection>
-[[Category: Burkart, M.D]]
+[[Category: Atlantibacter hermannii NBRC 105704]]
-[[Category: Sekhon, A]]
+[[Category: Escherichia coli K-12]]
-[[Category: Jiang, Z]]
+[[Category: Large Structures]]
+[[Category: Burkart MD]]
+[[Category: Chen A]]
+[[Category: Chen J]]
+[[Category: Jiang Z]]
+[[Category: La Clair JJ]]
+[[Category: Louie GV]]
+[[Category: Noel JP]]
+[[Category: Sekhon A]]