8cif: Difference between revisions
New page: '''Unreleased structure''' The entry 8cif is ON HOLD Authors: Clarke, J.D., Mikolajek, H., Stuart, D.I., Owens, R.J. Description: Bovine naive ultralong antibody AbD08 collected at 293... |
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The | ==Bovine naive ultralong antibody AbD08 collected at 293K== | ||
<StructureSection load='8cif' size='340' side='right'caption='[[8cif]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8cif]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CIF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cif OCA], [https://pdbe.org/8cif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cif RCSB], [https://www.ebi.ac.uk/pdbsum/8cif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cif ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The utility of X-ray crystal structures determined under ambient-temperature conditions is becoming increasingly recognized. Such experiments can allow protein dynamics to be characterized and are particularly well suited to challenging protein targets that may form fragile crystals that are difficult to cryo-cool. Room-temperature data collection also enables time-resolved experiments. In contrast to the high-throughput highly automated pipelines for determination of structures at cryogenic temperatures widely available at synchrotron beamlines, room-temperature methodology is less mature. Here, the current status of the fully automated ambient-temperature beamline VMXi at Diamond Light Source is described, and a highly efficient pipeline from protein sample to final multi-crystal data analysis and structure determination is shown. The capability of the pipeline is illustrated using a range of user case studies representing different challenges, and from high and lower symmetry space groups and varied crystal sizes. It is also demonstrated that very rapid structure determination from crystals in situ within crystallization plates is now routine with minimal user intervention. | |||
Protein-to-structure pipeline for ambient-temperature in situ crystallography at VMXi.,Mikolajek H, Sanchez-Weatherby J, Sandy J, Gildea RJ, Campeotto I, Cheruvara H, Clarke JD, Foster T, Fujii S, Paulsen IT, Shah BS, Hough MA IUCrJ. 2023 Jul 1;10(Pt 4):420-429. doi: 10.1107/S2052252523003810. PMID:37199504<ref>PMID:37199504</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Clarke | <div class="pdbe-citations 8cif" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Bos taurus]] | |||
[[Category: Large Structures]] | |||
[[Category: Clarke JD]] | |||
[[Category: Mikolajek H]] | |||
[[Category: Owens RJ]] | |||
[[Category: Stuart DI]] | |||