8c88: Difference between revisions

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-'''Unreleased structure'''
-The entry 8c88 is ON HOLD
+==Double mutant G(M19)C/T(L214)C structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV==
+<StructureSection load='8c88' size='340' side='right'caption='[[8c88]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8c88]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides_2.4.1 Cereibacter sphaeroides 2.4.1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C88 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=NKP:(2R)-2-HYDROXY-3-(PHOSPHONOOXY)PROPYL+(9E)-OCTADEC-9-ENOATE'>NKP</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c88 OCA], [https://pdbe.org/8c88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c88 RCSB], [https://www.ebi.ac.uk/pdbsum/8c88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c88 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The photosynthetic reaction center of the purple nonsulfur bacterium Cereibacter sphaeroides is a useful model for the study of mechanisms of photoinduced electron transfer and a promising component for photo-bio-electrocatalytic systems. The basic research and technological applications of this membrane pigment-protein complex require effective approaches to increase its structural stability. In this work, a rational design approach to genetically modify the reaction centers by introducing disulfide bonds is used. This resulted in significantly increasing the thermal stability of some of the mutant pigment-protein complexes. The formation of the S-S bonds was confirmed by X-ray crystallography as well as SDS-PAGE, and the optical properties of the reaction centers were studied. The genetically modified reaction centers presented here preserved their ability for photochemical charge separation and could be of interest for basic science and biotechnology.
-Authors: Gabdulkhakov, A.G., Selikhanov, G.K., Fufina, T.Y., Vasilieva, L.G., Atamas, A.A., Yukhimchuk, D.O.
+Stabilization of Cereibacter sphaeroides Photosynthetic Reaction Center by the Introduction of Disulfide Bonds.,Selikhanov G, Atamas A, Yukhimchuk D, Fufina T, Vasilieva L, Gabdulkhakov A Membranes (Basel). 2023 Jan 25;13(2):154. doi: 10.3390/membranes13020154. PMID:36837657<ref>PMID:36837657</ref>
-Description: Double mutant G(M19)C/T(L214)C structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Vasilieva, L.G]]
+<div class="pdbe-citations 8c88" style="background-color:#fffaf0;"></div>
-[[Category: Yukhimchuk, D.O]]
+== References ==
-[[Category: Gabdulkhakov, A.G]]
+<references/>
-[[Category: Atamas, A.A]]
+__TOC__
-[[Category: Selikhanov, G.K]]
+</StructureSection>
-[[Category: Fufina, T.Y]]
+[[Category: Cereibacter sphaeroides 2 4.1]]
+[[Category: Large Structures]]
+[[Category: Atamas A]]
+[[Category: Fufina T]]
+[[Category: Gabdulkhakov A]]
+[[Category: Selikhanov G]]
+[[Category: Vasilieva L]]
+[[Category: Yukhimchuk D]]