8btr: Difference between revisions
New page: '''Unreleased structure''' The entry 8btr is ON HOLD Authors: Majumdar, S., Emmerich, A.G., Sanyal, S. Description: Giardia Ribosome in PRE-T Hybrid State (D2) [[Category: Unreleased S... |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Giardia Ribosome in PRE-T Hybrid State (D2)== | |||
<StructureSection load='8btr' size='340' side='right'caption='[[8btr]], [[Resolution|resolution]] 3.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8btr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BTR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.25Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8btr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8btr OCA], [https://pdbe.org/8btr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8btr RCSB], [https://www.ebi.ac.uk/pdbsum/8btr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8btr ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/E2RTN4_GIAIC E2RTN4_GIAIC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Giardia intestinalis is a protozoan parasite that causes diarrhea in humans. Using single-particle cryo-electron microscopy, we have determined high-resolution structures of six naturally populated translocation intermediates, from ribosomes isolated directly from actively growing Giardia cells. The highly compact and uniquely GC-rich Giardia ribosomes possess eukaryotic rRNAs and ribosomal proteins, but retain some bacterial features. The translocation intermediates, with naturally bound tRNAs and eukaryotic elongation factor 2 (eEF2), display characteristic ribosomal intersubunit rotation and small subunit's head swiveling-universal for translocation. In addition, we observe the eukaryote-specific 'subunit rolling' dynamics, albeit with limited features. Finally, the eEF2.GDP state features a uniquely positioned 'leaving phosphate (Pi)' that proposes hitherto unknown molecular events of Pi and eEF2 release from the ribosome at the final stage of translocation. In summary, our study elucidates the mechanism of translocation in the protists and illustrates evolution of the translation machinery from bacteria to eukaryotes from both the structural and mechanistic perspectives. | |||
Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.,Majumdar S, Emmerich A, Krakovka S, Mandava CS, Svard SG, Sanyal S Nucleic Acids Res. 2023 Apr 24;51(7):3436-3451. doi: 10.1093/nar/gkad176. PMID:36912103<ref>PMID:36912103</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8btr" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Giardia lamblia ATCC 50803]] | |||
[[Category: Large Structures]] | |||
[[Category: Emmerich AG]] | |||
[[Category: Majumdar S]] | |||
[[Category: Sanyal S]] | |||
Latest revision as of 14:56, 23 October 2024
Giardia Ribosome in PRE-T Hybrid State (D2)Giardia Ribosome in PRE-T Hybrid State (D2)
Structural highlights
FunctionPublication Abstract from PubMedGiardia intestinalis is a protozoan parasite that causes diarrhea in humans. Using single-particle cryo-electron microscopy, we have determined high-resolution structures of six naturally populated translocation intermediates, from ribosomes isolated directly from actively growing Giardia cells. The highly compact and uniquely GC-rich Giardia ribosomes possess eukaryotic rRNAs and ribosomal proteins, but retain some bacterial features. The translocation intermediates, with naturally bound tRNAs and eukaryotic elongation factor 2 (eEF2), display characteristic ribosomal intersubunit rotation and small subunit's head swiveling-universal for translocation. In addition, we observe the eukaryote-specific 'subunit rolling' dynamics, albeit with limited features. Finally, the eEF2.GDP state features a uniquely positioned 'leaving phosphate (Pi)' that proposes hitherto unknown molecular events of Pi and eEF2 release from the ribosome at the final stage of translocation. In summary, our study elucidates the mechanism of translocation in the protists and illustrates evolution of the translation machinery from bacteria to eukaryotes from both the structural and mechanistic perspectives. Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.,Majumdar S, Emmerich A, Krakovka S, Mandava CS, Svard SG, Sanyal S Nucleic Acids Res. 2023 Apr 24;51(7):3436-3451. doi: 10.1093/nar/gkad176. PMID:36912103[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||