8b5r: Difference between revisions
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The | ==p97-p37-SPI substrate complex== | ||
<StructureSection load='8b5r' size='340' side='right'caption='[[8b5r]], [[Resolution|resolution]] 6.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8b5r]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B5R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B5R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.1Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b5r OCA], [https://pdbe.org/8b5r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b5r RCSB], [https://www.ebi.ac.uk/pdbsum/8b5r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b5r ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PP1R7_HUMAN PP1R7_HUMAN] Regulatory subunit of protein phosphatase 1. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The AAA+-ATPase p97 (also called VCP or Cdc48) unfolds proteins and disassembles protein complexes in numerous cellular processes, but how substrate complexes are loaded onto p97 and disassembled is unclear. Here, we present cryo-EM structures of p97 in the process of disassembling a protein phosphatase-1 (PP1) complex by extracting an inhibitory subunit from PP1. We show that PP1 and its partners SDS22 and inhibitor-3 (I3) are loaded tightly onto p97, surprisingly via a direct contact of SDS22 with the p97 N-domain. Loading is assisted by the p37 adapter that bridges two adjacent p97 N-domains underneath the substrate complex. A stretch of I3 is threaded into the central channel of the spiral-shaped p97 hexamer, while other elements of I3 are still attached to PP1. Thus, our data show how p97 arranges a protein complex between the p97 N-domain and central channel, suggesting a hold-and-extract mechanism for p97-mediated disassembly. | |||
Structural basis of ubiquitin-independent PP1 complex disassembly by p97.,van den Boom J, Marini G, Meyer H, Saibil HR EMBO J. 2023 Jul 17;42(14):e113110. doi: 10.15252/embj.2022113110. Epub 2023 Jun , 2. PMID:37264685<ref>PMID:37264685</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8b5r" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Van | __TOC__ | ||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Marini G]] | |||
[[Category: Meyer H]] | |||
[[Category: Saibil H]] | |||
[[Category: Van den Boom J]] | |||
Latest revision as of 09:47, 24 July 2024
p97-p37-SPI substrate complexp97-p37-SPI substrate complex
Structural highlights
FunctionPP1R7_HUMAN Regulatory subunit of protein phosphatase 1. Publication Abstract from PubMedThe AAA+-ATPase p97 (also called VCP or Cdc48) unfolds proteins and disassembles protein complexes in numerous cellular processes, but how substrate complexes are loaded onto p97 and disassembled is unclear. Here, we present cryo-EM structures of p97 in the process of disassembling a protein phosphatase-1 (PP1) complex by extracting an inhibitory subunit from PP1. We show that PP1 and its partners SDS22 and inhibitor-3 (I3) are loaded tightly onto p97, surprisingly via a direct contact of SDS22 with the p97 N-domain. Loading is assisted by the p37 adapter that bridges two adjacent p97 N-domains underneath the substrate complex. A stretch of I3 is threaded into the central channel of the spiral-shaped p97 hexamer, while other elements of I3 are still attached to PP1. Thus, our data show how p97 arranges a protein complex between the p97 N-domain and central channel, suggesting a hold-and-extract mechanism for p97-mediated disassembly. Structural basis of ubiquitin-independent PP1 complex disassembly by p97.,van den Boom J, Marini G, Meyer H, Saibil HR EMBO J. 2023 Jul 17;42(14):e113110. doi: 10.15252/embj.2022113110. Epub 2023 Jun , 2. PMID:37264685[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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