8h9j: Difference between revisions
New page: '''Unreleased structure''' The entry 8h9j is ON HOLD Authors: Lai, Y., Zhang, Y., Liu, F., Gao, Y., Gong, H., Rao, Z. Description: Human ATP synthase Fo domain, state2 [[Category: Unre... |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Human ATP synthase state2 subregion 3== | ||
<StructureSection load='8h9j' size='340' side='right'caption='[[8h9j]], [[Resolution|resolution]] 3.26Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8h9j]] is a 17 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H9J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H9J FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.26Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h9j OCA], [https://pdbe.org/8h9j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h9j RCSB], [https://www.ebi.ac.uk/pdbsum/8h9j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h9j ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/AT5G1_HUMAN AT5G1_HUMAN] Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Biological energy currency ATP is produced by F(1)F(o)-ATP synthase. However, the molecular mechanism for human ATP synthase action remains unknown. Here, we present snapshot images for three main rotational states and one substate of human ATP synthase using cryoelectron microscopy. These structures reveal that the release of ADP occurs when the beta subunit of F(1)F(o)-ATP synthase is in the open conformation, showing how ADP binding is coordinated during synthesis. The accommodation of the symmetry mismatch between F(1) and F(o) motors is resolved by the torsional flexing of the entire complex, especially the gamma subunit, and the rotational substep of the c subunit. Water molecules are identified in the inlet and outlet half-channels, suggesting that the proton transfer in these two half-channels proceed via a Grotthus mechanism. Clinically relevant mutations are mapped to the structure, showing that they are mainly located at the subunit-subunit interfaces, thus causing instability of the complex. | |||
Structure of the human ATP synthase.,Lai Y, Zhang Y, Zhou S, Xu J, Du Z, Feng Z, Yu L, Zhao Z, Wang W, Tang Y, Yang X, Guddat LW, Liu F, Gao Y, Rao Z, Gong H Mol Cell. 2023 Jun 15;83(12):2137-2147.e4. doi: 10.1016/j.molcel.2023.04.029. , Epub 2023 May 26. PMID:37244256<ref>PMID:37244256</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8h9j" style="background-color:#fffaf0;"></div> | ||
[[Category: Gao | == References == | ||
[[Category: | <references/> | ||
[[Category: Liu | __TOC__ | ||
[[Category: Rao | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | |||
[[Category: Gao Y]] | |||
[[Category: Gong H]] | |||
[[Category: Lai Y]] | |||
[[Category: Liu F]] | |||
[[Category: Rao Z]] | |||
[[Category: Zhang Y]] | |||
Latest revision as of 10:37, 3 July 2024
Human ATP synthase state2 subregion 3Human ATP synthase state2 subregion 3
Structural highlights
FunctionAT5G1_HUMAN Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. Publication Abstract from PubMedBiological energy currency ATP is produced by F(1)F(o)-ATP synthase. However, the molecular mechanism for human ATP synthase action remains unknown. Here, we present snapshot images for three main rotational states and one substate of human ATP synthase using cryoelectron microscopy. These structures reveal that the release of ADP occurs when the beta subunit of F(1)F(o)-ATP synthase is in the open conformation, showing how ADP binding is coordinated during synthesis. The accommodation of the symmetry mismatch between F(1) and F(o) motors is resolved by the torsional flexing of the entire complex, especially the gamma subunit, and the rotational substep of the c subunit. Water molecules are identified in the inlet and outlet half-channels, suggesting that the proton transfer in these two half-channels proceed via a Grotthus mechanism. Clinically relevant mutations are mapped to the structure, showing that they are mainly located at the subunit-subunit interfaces, thus causing instability of the complex. Structure of the human ATP synthase.,Lai Y, Zhang Y, Zhou S, Xu J, Du Z, Feng Z, Yu L, Zhao Z, Wang W, Tang Y, Yang X, Guddat LW, Liu F, Gao Y, Rao Z, Gong H Mol Cell. 2023 Jun 15;83(12):2137-2147.e4. doi: 10.1016/j.molcel.2023.04.029. , Epub 2023 May 26. PMID:37244256[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||