8gv8: Difference between revisions
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The | ==The cryo-EM structure of hAE2 with DIDS== | ||
<StructureSection load='8gv8' size='340' side='right'caption='[[8gv8]], [[Resolution|resolution]] 3.08Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8gv8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GV8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.08Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4KU:2,2-ETHANE-1,2-DIYLBIS{5-[(SULFANYLMETHYL)AMINO]BENZENESULFONIC+ACID}'>4KU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gv8 OCA], [https://pdbe.org/8gv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gv8 RCSB], [https://www.ebi.ac.uk/pdbsum/8gv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gv8 ProSAT]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[https://www.uniprot.org/uniprot/B3A2_HUMAN B3A2_HUMAN] The disease may be caused by variants affecting the gene represented in this entry. | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B3A2_HUMAN B3A2_HUMAN] Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed:15184086, PubMed:34668226). Plays an important role in osteoclast differentiation and function (PubMed:34668226). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (By similarity). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (By similarity).[UniProtKB:P13808]<ref>PMID:15184086</ref> <ref>PMID:34668226</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cell maintains its intracellular pH in a narrow physiological range and disrupting the pH-homeostasis could cause dysfunctional metabolic states. Anion exchanger 2 (AE2) works at high cellular pH to catalyze the exchange between the intracellular HCO(3)(-) and extracellular Cl(-), thereby maintaining the pH-homeostasis. Here, we determine the cryo-EM structures of human AE2 in five major operating states and one transitional hybrid state. Among those states, the AE2 shows the inward-facing, outward-facing, and intermediate conformations, as well as the substrate-binding pockets at two sides of the cell membrane. Furthermore, critical structural features were identified showing an interlock mechanism for interactions among the cytoplasmic N-terminal domain and the transmembrane domain and the self-inhibitory effect of the C-terminal loop. The structural and cell-based functional assay collectively demonstrate the dynamic process of the anion exchange across membranes and provide the structural basis for the pH-sensitive pH-rebalancing activity of AE2. | |||
The structural basis of the pH-homeostasis mediated by the Cl(-)/HCO(3)(-) exchanger, AE2.,Zhang Q, Jian L, Yao D, Rao B, Xia Y, Hu K, Li S, Shen Y, Cao M, Qin A, Zhao J, Cao Y Nat Commun. 2023 Mar 31;14(1):1812. doi: 10.1038/s41467-023-37557-y. PMID:37002221<ref>PMID:37002221</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8gv8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Anion exchange protein 3D structures|Anion exchange protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Cao Y]] | |||
[[Category: Hu K]] | |||
[[Category: Jian L]] | |||
[[Category: Rao B]] | |||
[[Category: Xia Y]] | |||
[[Category: Yao D]] | |||
[[Category: Zhang Q]] | |||
Latest revision as of 17:37, 6 November 2024
The cryo-EM structure of hAE2 with DIDSThe cryo-EM structure of hAE2 with DIDS
Structural highlights
DiseaseB3A2_HUMAN The disease may be caused by variants affecting the gene represented in this entry. FunctionB3A2_HUMAN Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed:15184086, PubMed:34668226). Plays an important role in osteoclast differentiation and function (PubMed:34668226). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (By similarity). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (By similarity).[UniProtKB:P13808][1] [2] Publication Abstract from PubMedThe cell maintains its intracellular pH in a narrow physiological range and disrupting the pH-homeostasis could cause dysfunctional metabolic states. Anion exchanger 2 (AE2) works at high cellular pH to catalyze the exchange between the intracellular HCO(3)(-) and extracellular Cl(-), thereby maintaining the pH-homeostasis. Here, we determine the cryo-EM structures of human AE2 in five major operating states and one transitional hybrid state. Among those states, the AE2 shows the inward-facing, outward-facing, and intermediate conformations, as well as the substrate-binding pockets at two sides of the cell membrane. Furthermore, critical structural features were identified showing an interlock mechanism for interactions among the cytoplasmic N-terminal domain and the transmembrane domain and the self-inhibitory effect of the C-terminal loop. The structural and cell-based functional assay collectively demonstrate the dynamic process of the anion exchange across membranes and provide the structural basis for the pH-sensitive pH-rebalancing activity of AE2. The structural basis of the pH-homeostasis mediated by the Cl(-)/HCO(3)(-) exchanger, AE2.,Zhang Q, Jian L, Yao D, Rao B, Xia Y, Hu K, Li S, Shen Y, Cao M, Qin A, Zhao J, Cao Y Nat Commun. 2023 Mar 31;14(1):1812. doi: 10.1038/s41467-023-37557-y. PMID:37002221[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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