7ygc: Difference between revisions

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-'''Unreleased structure'''
-The entry 7ygc is ON HOLD  until Paper Publication
+==Cryo-EM structure of Tetrahymena ribozyme conformation 4 undergoing the second-step self-splicing==
+<StructureSection load='7ygc' size='340' side='right'caption='[[7ygc]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7ygc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YGC FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSU:URIDINE-5-PHOSPHOROTHIOATE'>SSU</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ygc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ygc OCA], [https://pdbe.org/7ygc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ygc RCSB], [https://www.ebi.ac.uk/pdbsum/7ygc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ygc ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing.
-Authors:
+Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.,Li S, Palo MZ, Zhang X, Pintilie G, Zhang K Nat Commun. 2023 Mar 16;14(1):1294. doi: 10.1038/s41467-023-36724-5. PMID:36928031<ref>PMID:36928031</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7ygc" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ribozyme 3D structures|Ribozyme 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Tetrahymena thermophila]]
+[[Category: Greg P]]
+[[Category: Li S]]
+[[Category: Michael ZP]]
+[[Category: Zhang K]]
+[[Category: Zhang X]]