8a9x: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8a9x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_oxytoca Klebsiella oxytoca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9X FirstGlance]. <br>
<table><tr><td colspan='2'>[[8a9x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_oxytoca Klebsiella oxytoca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9X FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9x OCA], [https://pdbe.org/8a9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9x RCSB], [https://www.ebi.ac.uk/pdbsum/8a9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9x ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.523&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9x OCA], [https://pdbe.org/8a9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9x RCSB], [https://www.ebi.ac.uk/pdbsum/8a9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9x ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/GSPM_KLEPN GSPM_KLEPN] Inner membrane component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Plays a role in the complex assembly and recruits PulL resulting in a stable complex in the inner membrane. Provides thus a link between the energy-providing PulE protein in the cytoplasm and the rest of the T2SS machinery.[UniProtKB:P25061]
Type II secretion systems (T2SSs) allow diderm bacteria to secrete hydrolytic enzymes, adhesins, or toxins important for growth and virulence. To promote secretion of folded proteins, T2SSs assemble periplasmic filaments called pseudopili or endopili at an inner membrane subcomplex, the assembly platform (AP). Here, we combined biophysical approaches, nuclear magnetic resonance (NMR) and X-ray crystallography, to study the Klebsiella AP components PulL and PulM. We determined the structure and associations of their periplasmic domains and describe the structure of the heterodimer formed by their ferredoxin-like domains. We show how structural complementarity and plasticity favor their association during the secretion process. Cysteine scanning and crosslinking data provided additional constraints to build a structural model of the PulL-PulM assembly in the cellular context. Our structural and functional insights, together with the relative cellular abundance of its components, support the role of AP as a dynamic hub that orchestrates pilus polymerization.


Structure and dynamic association of an assembly platform subcomplex of the bacterial type II secretion system.,Dazzoni R, Li Y, Lopez-Castilla A, Brier S, Mechaly A, Cordier F, Haouz A, Nilges M, Francetic O, Bardiaux B, Izadi-Pruneyre N Structure. 2022 Dec 16:S0969-2126(22)00489-0. doi: 10.1016/j.str.2022.12.003. PMID:36586404<ref>PMID:36586404</ref>
==See Also==
 
*[[General secretion pathway protein 3D structures|General secretion pathway protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8a9x" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Francetic O]]
[[Category: Francetic O]]
[[Category: Haouz A]]
[[Category: Haouz A]]
[[Category: Izadi N]]
[[Category: Izadi-Pruneyre N]]
[[Category: Li Y]]
[[Category: Li Y]]
[[Category: Lopez-Castilla A]]
[[Category: Lopez-Castilla A]]
[[Category: Mechaly A]]
[[Category: Mechaly A]]
[[Category: Nilges M]]
[[Category: Nilges M]]

Latest revision as of 09:24, 19 June 2024

Crystal structure of PulM C-ter domainCrystal structure of PulM C-ter domain

Structural highlights

8a9x is a 7 chain structure with sequence from Klebsiella oxytoca. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.523Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GSPM_KLEPN Inner membrane component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Plays a role in the complex assembly and recruits PulL resulting in a stable complex in the inner membrane. Provides thus a link between the energy-providing PulE protein in the cytoplasm and the rest of the T2SS machinery.[UniProtKB:P25061]

See Also

8a9x, resolution 1.52Å

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