8dfv: Difference between revisions
New page: '''Unreleased structure''' The entry 8dfv is ON HOLD Authors: Jouravleva, K., Golovenko, D., Demo, G., Dutcher, R.C., Tanaka Hall, T.M., Zamore, P.D., Korostelev, A.A. Description: Str... |
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==Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIa== | |||
<StructureSection load='8dfv' size='340' side='right'caption='[[8dfv]], [[Resolution|resolution]] 3.06Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8dfv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DFV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.06Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfv OCA], [https://pdbe.org/8dfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfv RCSB], [https://www.ebi.ac.uk/pdbsum/8dfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfv ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner LoqsâPB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicerâ1â
LoqsâPB heterodimer. The Dicer-1 dsRBD and three LoqsâPB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicerâ1â
LoqsâPB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release. | |||
Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.,Jouravleva K, Golovenko D, Demo G, Dutcher RC, Hall TMT, Zamore PD, Korostelev AA Mol Cell. 2022 Nov 3;82(21):4049-4063.e6. doi: 10.1016/j.molcel.2022.09.002. Epub , 2022 Sep 30. PMID:36182693<ref>PMID:36182693</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8dfv" style="background-color:#fffaf0;"></div> | ||
[[Category: Demo | == References == | ||
[[Category: Golovenko | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Drosophila melanogaster]] | ||
[[Category: Zamore | [[Category: Large Structures]] | ||
[[Category: Demo G]] | |||
[[Category: Dutcher RC]] | |||
[[Category: Golovenko D]] | |||
[[Category: Jouravleva K]] | |||
[[Category: Korostelev AA]] | |||
[[Category: Tanaka Hall TM]] | |||
[[Category: Zamore PD]] | |||
Latest revision as of 08:21, 12 June 2024
Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIaStructural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIa
Structural highlights
Publication Abstract from PubMedIn animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner LoqsâPB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicerâ1â LoqsâPB heterodimer. The Dicer-1 dsRBD and three LoqsâPB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicerâ1â LoqsâPB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release. Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.,Jouravleva K, Golovenko D, Demo G, Dutcher RC, Hall TMT, Zamore PD, Korostelev AA Mol Cell. 2022 Nov 3;82(21):4049-4063.e6. doi: 10.1016/j.molcel.2022.09.002. Epub , 2022 Sep 30. PMID:36182693[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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