7y5h: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Unreleased structure'''
-The entry 7y5h is ON HOLD
+==Cryo-EM structure of a eukaryotic ZnT8 at a low pH==
+<StructureSection load='7y5h' size='340' side='right'caption='[[7y5h]], [[Resolution|resolution]] 3.72&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7y5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_tropicalis Xenopus tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y5H FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.72&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y5h OCA], [https://pdbe.org/7y5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y5h RCSB], [https://www.ebi.ac.uk/pdbsum/7y5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y5h ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ZNT8_XENTR ZNT8_XENTR] Proton-coupled zinc ion antiporter mediating the entry of zinc into the lumen of pancreatic beta cell secretory granules, thereby regulating insulin secretion.[UniProtKB:Q8IWU4]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Zinc transporter 8 (ZnT8) is mainly expressed in pancreatic islet beta cells and is responsible for H(+)-coupled uptake (antiport) of Zn(2+) into the lumen of insulin secretory granules. Structures of human ZnT8 and its prokaryotic homolog YiiP have provided structural basis for constructing a plausible transport cycle for Zn(2+). However, the mechanistic role that protons play in the transport process remains unclear. Here we present a lumen-facing cryo-EM structure of ZnT8 from Xenopus tropicalis (xtZnT8) in the presence of Zn(2+) at a luminal pH (5.5). Compared to a Zn(2+)-bound xtZnT8 structure at a cytosolic pH (7.5), the low-pH structure displays an empty transmembrane Zn(2+)-binding site with a disrupted coordination geometry. Combined with a Zn(2+)-binding assay our data suggest that protons may disrupt Zn(2+) coordination at the transmembrane Zn(2+)-binding site in the lumen-facing state, thus facilitating Zn(2+) release from ZnT8 into the lumen.
-Authors: Zhang, S., Fu, C., Luo, Y., Sun, Z., Su, Z., Zhou, X.
+Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn(2+)-releasing mechanism.,Zhang S, Fu C, Luo Y, Xie Q, Xu T, Sun Z, Su Z, Zhou X J Struct Biol. 2023 Mar;215(1):107926. doi: 10.1016/j.jsb.2022.107926. Epub 2022 , Dec 1. PMID:36464198<ref>PMID:36464198</ref>
-Description: Cryo-EM structure of a eukaryotic ZnT8 at a low pH
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Zhou, X]]
+<div class="pdbe-citations 7y5h" style="background-color:#fffaf0;"></div>
-[[Category: Su, Z]]
+== References ==
-[[Category: Luo, Y]]
+<references/>
-[[Category: Zhang, S]]
+__TOC__
-[[Category: Fu, C]]
+</StructureSection>
-[[Category: Sun, Z]]
+[[Category: Large Structures]]
+[[Category: Xenopus tropicalis]]
+[[Category: Fu C]]
+[[Category: Luo Y]]
+[[Category: Su Z]]
+[[Category: Sun Z]]
+[[Category: Zhang S]]
+[[Category: Zhou X]]