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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/ | [https://www.uniprot.org/uniprot/NQRA_VIBC3 NQRA_VIBC3] NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to NqrE are probably involved in the second step, the conversion of ubisemiquinone to ubiquinol.[HAMAP-Rule:MF_00425] | ||
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== Publication Abstract from PubMed == | |||
In the respiratory chain, NADH oxidation is coupled to ion translocation across the membrane to build up an electrochemical gradient. In the human pathogen Vibrio cholerae, the sodium-pumping NADH:quinone oxidoreductase (Na(+)-NQR) generates a sodium gradient by a so far unknown mechanism. Here we show that ion pumping in Na(+)-NQR is driven by large conformational changes coupling electron transfer to ion translocation. We have determined a series of cryo-EM and X-ray structures of the Na(+)-NQR that represent snapshots of the catalytic cycle. The six subunits NqrA, B, C, D, E, and F of Na(+)-NQR harbor a unique set of cofactors that shuttle the electrons from NADH twice across the membrane to quinone. The redox state of a unique intramembranous [2Fe-2S] cluster orchestrates the movements of subunit NqrC, which acts as an electron transfer switch. We propose that this switching movement controls the release of Na(+) from a binding site localized in subunit NqrB. | |||
Conformational coupling of redox-driven Na(+)-translocation in Vibrio cholerae NADH:quinone oxidoreductase.,Hau JL, Kaltwasser S, Muras V, Casutt MS, Vohl G, Claussen B, Steffen W, Leitner A, Bill E, Cutsail GE 3rd, DeBeer S, Vonck J, Steuber J, Fritz G Nat Struct Mol Biol. 2023 Nov;30(11):1686-1694. doi: 10.1038/s41594-023-01099-0. , Epub 2023 Sep 14. PMID:37710014<ref>PMID:37710014</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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==See Also== | ==See Also== | ||
*[[Quinone reductase 3D structures|Quinone reductase 3D structures]] | *[[Quinone reductase 3D structures|Quinone reductase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||