7xl3: Difference between revisions
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==== | ==Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe)== | ||
<StructureSection load='7xl3' size='340' side='right'caption='[[7xl3]]' scene=''> | <StructureSection load='7xl3' size='340' side='right'caption='[[7xl3]], [[Resolution|resolution]] 3.13Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7xl3]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XL3 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xl3 OCA], [https://pdbe.org/7xl3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xl3 RCSB], [https://www.ebi.ac.uk/pdbsum/7xl3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xl3 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.13Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xl3 OCA], [https://pdbe.org/7xl3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xl3 RCSB], [https://www.ebi.ac.uk/pdbsum/7xl3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xl3 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/RPOA_PSEAE RPOA_PSEAE] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_00059] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pseudomonas aeruginosa (Pae) SutA adapts bacteria to hypoxia and nutrition-limited environment during chronic infection by increasing transcription activity of an RNA polymerase (RNAP) holoenzyme comprising the stress-responsive sigma factor sigma(S) (RNAP-sigma(S)). SutA shows no homology to previously characterized RNAP-binding proteins. The structure and mode of action of SutA remain unclear. Here we determined cryo-EM structures of Pae RNAP-sigma(S) holoenzyme, Pae RNAP-sigma(S) holoenzyme complexed with SutA, and Pae RNAP-sigma(S) transcription initiation complex comprising SutA. The structures show SutA pinches RNAP-beta protrusion and facilitates promoter unwinding by wedging RNAP-beta lobe open. Our results demonstrate that SutA clears an energetic barrier to facilitate promoter unwinding of RNAP-sigma(S) holoenzyme. | |||
Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding.,He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y Nat Commun. 2022 Jul 20;13(1):4204. doi: 10.1038/s41467-022-31871-7. PMID:35859063<ref>PMID:35859063</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7xl3" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
*[[Sigma factor 3D structures|Sigma factor 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: He DW]] | |||
[[Category: You LL]] | |||
[[Category: Zhang Y]] | |||