7udc: Difference between revisions

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-'''Unreleased structure'''
-The entry 7udc is ON HOLD
+==cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex class1==
+<StructureSection load='7udc' size='340' side='right'caption='[[7udc]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7udc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UDC FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7udc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7udc OCA], [https://pdbe.org/7udc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7udc RCSB], [https://www.ebi.ac.uk/pdbsum/7udc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7udc ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Munc18-1 forms a template to organize assembly of the neuronal SNARE complex that triggers neurotransmitter release, binding first to a closed conformation of syntaxin-1 where its amino-terminal region interacts with the SNARE motif, and later binding to synaptobrevin. However, the mechanism of SNARE complex assembly remains unclear. Here, we report two cryo-EM structures of Munc18-1 bound to cross-linked syntaxin-1 and synaptobrevin. The structures allow visualization of how syntaxin-1 opens and reveal how part of the syntaxin-1 amino-terminal region can help nucleate interactions between the amino termini of the syntaxin-1 and synaptobrevin SNARE motifs, while their carboxyl termini bind to distal sites of Munc18-1. These observations, together with mutagenesis, SNARE complex assembly experiments, and fusion assays with reconstituted proteoliposomes, support a model whereby these interactions are critical to initiate SNARE complex assembly and multiple energy barriers enable diverse mechanisms for exquisite regulation of neurotransmitter release.
-Authors: Rizo, J., Bai, X., Stepien, K.P., Xu, J., Zhang, X.
+SNARE assembly enlightened by cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex.,Stepien KP, Xu J, Zhang X, Bai XC, Rizo J Sci Adv. 2022 Jun 24;8(25):eabo5272. doi: 10.1126/sciadv.abo5272. Epub 2022 Jun , 22. PMID:35731863<ref>PMID:35731863</ref>
-Description: cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex class1
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Rizo, J]]
+<div class="pdbe-citations 7udc" style="background-color:#fffaf0;"></div>
-[[Category: Bai, X]]
-[[Category: Xu, J]]
+==See Also==
-[[Category: Zhang, X]]
+*[[Syntaxin 3D structures|Syntaxin 3D structures]]
-[[Category: Stepien, K.P]]
+*[[Syntaxin-binding protein|Syntaxin-binding protein]]
+*[[Vesicle-associated membrane protein 3D structures|Vesicle-associated membrane protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Rattus norvegicus]]
+[[Category: Bai X]]
+[[Category: Rizo J]]
+[[Category: Stepien KP]]
+[[Category: Xu J]]
+[[Category: Zhang X]]