7z7q: Difference between revisions
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<StructureSection load='7z7q' size='340' side='right'caption='[[7z7q]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='7z7q' size='340' side='right'caption='[[7z7q]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7Q FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IO8:2-[2-(aminomethyl)-4-(1H-indol-3-ylmethyl)-5-oxidanylidene-4H-imidazol-1-yl]ethanoic+acid'>IO8</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IO8:2-[2-(aminomethyl)-4-(1H-indol-3-ylmethyl)-5-oxidanylidene-4H-imidazol-1-yl]ethanoic+acid'>IO8</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7q OCA], [https://pdbe.org/7z7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7q RCSB], [https://www.ebi.ac.uk/pdbsum/7z7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7q ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7q OCA], [https://pdbe.org/7z7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7q RCSB], [https://www.ebi.ac.uk/pdbsum/7z7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Duarte K]] | [[Category: Duarte K]] | ||
[[Category: Dupuy J]] | [[Category: Dupuy J]] | ||
[[Category: Royant A]] | [[Category: Royant A]] | ||
Latest revision as of 12:24, 17 October 2024
Structure of the T207D single-point mutant of the fluorescent protein NeonCyan1 at pH 6.5Structure of the T207D single-point mutant of the fluorescent protein NeonCyan1 at pH 6.5
Structural highlights
Publication Abstract from PubMedmNeonGreen, an engineered green fluorescent protein (GFP) derived from lancelet, is one of the most brightly fluorescent homologues of Aequorea victoria jellyfish GFP (avGFP) yet reported. In this work, we investigated whether this bright fluorescence might be retained in homologs of mNeonGreen with modified chromophore structures and altered fluorescent hues. We found mNeonGreen to be generally less tolerant than avGFP to chromophore modification by substitution of the key chromophore-forming tyrosine residue with other aromatic amino acids. However, we were ultimately successful in creating a variant, designated as NeonCyan1, with a tryptophan-derived cyan fluorescent protein (CFP)-type chromophore, and two additional mutants with distinct spectral hues. Structural, computational, and photophysical characterization of NeonCyan1 and its variants provided insight into the factors that control the fluorescence emission color. Though not recommended as replacements for contemporary CFP variants, we demonstrate that NeonCyan1 variants are potentially suitable for live cell imaging applications. Cyan fluorescent proteins derived from mNeonGreen.,Zarowny L, Clavel D, Johannson R, Duarte K, Depernet H, Dupuy J, Baker H, Brown A, Royant A, Campbell RE Protein Eng Des Sel. 2022 Apr 13. pii: 6567951. doi: 10.1093/protein/gzac004. PMID:35417013[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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