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==Structure of the fluorescent protein NeonCyan0.95 at pH 7.5== | ==Structure of the fluorescent protein NeonCyan0.95 at pH 7.5== | ||
<StructureSection load='7z7o' size='340' side='right'caption='[[7z7o]]' scene=''> | <StructureSection load='7z7o' size='340' side='right'caption='[[7z7o]], [[Resolution|resolution]] 1.51Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7O FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7O FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7o OCA], [https://pdbe.org/7z7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7o RCSB], [https://www.ebi.ac.uk/pdbsum/7z7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7o ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IO8:2-[2-(aminomethyl)-4-(1H-indol-3-ylmethyl)-5-oxidanylidene-4H-imidazol-1-yl]ethanoic+acid'>IO8</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7o OCA], [https://pdbe.org/7z7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7o RCSB], [https://www.ebi.ac.uk/pdbsum/7z7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7o ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
mNeonGreen, an engineered green fluorescent protein (GFP) derived from lancelet, is one of the most brightly fluorescent homologues of Aequorea victoria jellyfish GFP (avGFP) yet reported. In this work, we investigated whether this bright fluorescence might be retained in homologs of mNeonGreen with modified chromophore structures and altered fluorescent hues. We found mNeonGreen to be generally less tolerant than avGFP to chromophore modification by substitution of the key chromophore-forming tyrosine residue with other aromatic amino acids. However, we were ultimately successful in creating a variant, designated as NeonCyan1, with a tryptophan-derived cyan fluorescent protein (CFP)-type chromophore, and two additional mutants with distinct spectral hues. Structural, computational, and photophysical characterization of NeonCyan1 and its variants provided insight into the factors that control the fluorescence emission color. Though not recommended as replacements for contemporary CFP variants, we demonstrate that NeonCyan1 variants are potentially suitable for live cell imaging applications. | |||
Cyan fluorescent proteins derived from mNeonGreen.,Zarowny L, Clavel D, Johannson R, Duarte K, Depernet H, Dupuy J, Baker H, Brown A, Royant A, Campbell RE Protein Eng Des Sel. 2022 Apr 13. pii: 6567951. doi: 10.1093/protein/gzac004. PMID:35417013<ref>PMID:35417013</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7z7o" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 17:17, 6 November 2024
Structure of the fluorescent protein NeonCyan0.95 at pH 7.5Structure of the fluorescent protein NeonCyan0.95 at pH 7.5
Structural highlights
Publication Abstract from PubMedmNeonGreen, an engineered green fluorescent protein (GFP) derived from lancelet, is one of the most brightly fluorescent homologues of Aequorea victoria jellyfish GFP (avGFP) yet reported. In this work, we investigated whether this bright fluorescence might be retained in homologs of mNeonGreen with modified chromophore structures and altered fluorescent hues. We found mNeonGreen to be generally less tolerant than avGFP to chromophore modification by substitution of the key chromophore-forming tyrosine residue with other aromatic amino acids. However, we were ultimately successful in creating a variant, designated as NeonCyan1, with a tryptophan-derived cyan fluorescent protein (CFP)-type chromophore, and two additional mutants with distinct spectral hues. Structural, computational, and photophysical characterization of NeonCyan1 and its variants provided insight into the factors that control the fluorescence emission color. Though not recommended as replacements for contemporary CFP variants, we demonstrate that NeonCyan1 variants are potentially suitable for live cell imaging applications. Cyan fluorescent proteins derived from mNeonGreen.,Zarowny L, Clavel D, Johannson R, Duarte K, Depernet H, Dupuy J, Baker H, Brown A, Royant A, Campbell RE Protein Eng Des Sel. 2022 Apr 13. pii: 6567951. doi: 10.1093/protein/gzac004. PMID:35417013[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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