7z3v: Difference between revisions
New page: '''Unreleased structure''' The entry 7z3v is ON HOLD until sometime in the future Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M. Description: Escherichia coli periplasmic ... |
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==Escherichia coli periplasmic phytase AppA D304E mutant, complex with myo-inositol hexakissulfate== | |||
<StructureSection load='7z3v' size='340' side='right'caption='[[7z3v]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7z3v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z3V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z3v OCA], [https://pdbe.org/7z3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z3v RCSB], [https://www.ebi.ac.uk/pdbsum/7z3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z3v ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PPA_ECOLI PPA_ECOLI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
AppA, the Escherichia coli periplasmic phytase of clade 2 of the histidine phosphatase (HP2) family, has been well-characterized and successfully engineered for use as an animal feed supplement. AppA is a 1D-6-phytase and highly stereospecific but transiently accumulates 1D-myo-Ins(2,3,4,5)P(4) and other lower phosphorylated intermediates. If this bottleneck in liberation of orthophosphate is to be obviated through protein engineering, an explanation of its rather rigid preference for the initial site and subsequent cleavage of phytic acid is required. To help explain this behaviour, the role of the catalytic proton donor residue in determining AppA stereospecificity was investigated. Four variants were generated by site-directed mutagenesis of the active site HDT amino acid sequence motif containing the catalytic proton donor, D304. The identity and position of the prospective proton donor residue was found to strongly influence stereospecificity. While the wild-type enzyme has a strong preference for 1D-6-phytase activity, a marked reduction in stereospecificity was observed for a D304E variant, while a proton donor-less mutant (D304A) displayed exclusive 1D-1/3-phytase activity. High-resolution X-ray crystal structures of complexes of the mutants with a non-hydrolysable substrate analogue inhibitor point to a crucial role played by D304 in stereospecificity by influencing the size and polarity of specificity pockets A and B. Taken together, these results provide the first evidence for the involvement of the proton donor residue in determining the stereospecificity of HP2 phytases and prepares the ground for structure-informed engineering studies targeting the production of animal feed enzymes capable of the efficient and complete dephosphorylation of dietary phytic acid. | |||
Insights to the Structural Basis for the Stereospecificity of the Escherichia coli Phytase, AppA.,Acquistapace IM, Thompson EJ, Kuhn I, Bedford MR, Brearley CA, Hemmings AM Int J Mol Sci. 2022 Jun 6;23(11):6346. doi: 10.3390/ijms23116346. PMID:35683026<ref>PMID:35683026</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7z3v" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Hemmings | ==See Also== | ||
*[[Acid phosphatase 3D structures|Acid phosphatase 3D structures]] | |||
*[[Phytase 3D structures|Phytase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | |||
[[Category: Large Structures]] | |||
[[Category: Acquistapace IM]] | |||
[[Category: Brearley CA]] | |||
[[Category: Hemmings AM]] | |||