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==== | ==Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA== | ||
<StructureSection load='7z0o' size='340' side='right'caption='[[7z0o]]' scene=''> | <StructureSection load='7z0o' size='340' side='right'caption='[[7z0o]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7z0o]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z0O FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z0o OCA], [https://pdbe.org/7z0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z0o RCSB], [https://www.ebi.ac.uk/pdbsum/7z0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z0o ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z0o OCA], [https://pdbe.org/7z0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z0o RCSB], [https://www.ebi.ac.uk/pdbsum/7z0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z0o ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/H3_YEAST H3_YEAST] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Preribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I preinitiation complex (PIC) formation at the 35S ribosomal RNA gene. To visualize the molecular intermediates toward PIC formation, we determined the structure of UAF in complex with native promoter DNA and transcription factor TATA-box-binding protein (TBP). We found that UAF recognizes DNA using a hexameric histone-like scaffold with markedly different interactions compared with the nucleosome and the histone-fold-rich transcription factor IID (TFIID). In parallel, UAF positions TBP for Core Factor binding, which leads to Pol I recruitment, while sequestering it from DNA and Pol II/III-specific transcription factors. Our work thus reveals the structural basis of RNA Pol selection by a transcription factor. | |||
Mechanism of RNA polymerase I selection by transcription factor UAF.,Baudin F, Murciano B, Fung HKH, Fromm SA, Mattei S, Mahamid J, Muller CW Sci Adv. 2022 Apr 22;8(16):eabn5725. doi: 10.1126/sciadv.abn5725. Epub 2022 Apr , 20. PMID:35442737<ref>PMID:35442737</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7z0o" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Histone 3D structures|Histone 3D structures]] | |||
*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Baudin F]] | |||
[[Category: Fromm SA]] | |||
[[Category: Fung HKH]] | |||
[[Category: Mueller CW]] | |||
[[Category: Murciano B]] | |||
Latest revision as of 15:40, 17 July 2024
Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNAStructure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA
Structural highlights
FunctionPublication Abstract from PubMedPreribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I preinitiation complex (PIC) formation at the 35S ribosomal RNA gene. To visualize the molecular intermediates toward PIC formation, we determined the structure of UAF in complex with native promoter DNA and transcription factor TATA-box-binding protein (TBP). We found that UAF recognizes DNA using a hexameric histone-like scaffold with markedly different interactions compared with the nucleosome and the histone-fold-rich transcription factor IID (TFIID). In parallel, UAF positions TBP for Core Factor binding, which leads to Pol I recruitment, while sequestering it from DNA and Pol II/III-specific transcription factors. Our work thus reveals the structural basis of RNA Pol selection by a transcription factor. Mechanism of RNA polymerase I selection by transcription factor UAF.,Baudin F, Murciano B, Fung HKH, Fromm SA, Mattei S, Mahamid J, Muller CW Sci Adv. 2022 Apr 22;8(16):eabn5725. doi: 10.1126/sciadv.abn5725. Epub 2022 Apr , 20. PMID:35442737[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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