7vy6: Difference between revisions
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==== | ==Coxsackievirus B3(VP3-234N) incubate with CD55 at pH7.4== | ||
<StructureSection load='7vy6' size='340' side='right'caption='[[7vy6]]' scene=''> | <StructureSection load='7vy6' size='340' side='right'caption='[[7vy6]], [[Resolution|resolution]] 3.02Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7vy6]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxsackievirus_B3 Coxsackievirus B3] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VY6 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vy6 OCA], [https://pdbe.org/7vy6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vy6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vy6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vy6 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.02Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vy6 OCA], [https://pdbe.org/7vy6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vy6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vy6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vy6 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Receptor usage defines cell tropism and contributes to cell entry and infection. Coxsackievirus B (CVB) engages coxsackievirus and adenovirus receptor (CAR), and selectively utilizes the decay-accelerating factor (DAF; CD55) to infect cells. However, the differential receptor usage mechanism for CVB remains elusive. This study identified VP3-234 residues (234Q/N/V/D/E) as critical population selection determinants during CVB3 virus evolution, contributing to diverse binding affinities to CD55. Cryoelectron microscopy (cryo-EM) structures of CD55-binding/nonbinding isolates and their complexes with CD55 or CAR were obtained under both neutral and acidic conditions, and the molecular mechanism of VP3-234 residues determining CD55 affinity/specificity for naturally occurring CVB3 strains was elucidated. Structural and biochemical studies in vitro revealed the dynamic entry process of CVB3 and the function of the uncoating receptor CAR with different pH preferences. This work provides detailed insight into the molecular mechanism of CVB infection and contributes to an in-depth understanding of enterovirus attachment receptor usage. | |||
Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains.,Wang Q, Yang Q, Liu C, Wang G, Song H, Shang G, Peng R, Qu X, Liu S, Cui Y, Wang P, Xu W, Zhao X, Qi J, Yang M, Gao GF Proc Natl Acad Sci U S A. 2022 Jan 25;119(4):e2118590119. doi: , 10.1073/pnas.2118590119. PMID:35046043<ref>PMID:35046043</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7vy6" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[CD55|CD55]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Coxsackievirus B3]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Liu CC]] | ||
[[Category: Wang QL]] | |||