7s6d: Difference between revisions
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New page: '''Unreleased structure''' The entry 7s6d is ON HOLD Authors: Bagde, S.R., Kim, C.-Y., Fromme, J.C. Description: CryoEM structure of modular PKS holo-Lsd14 bound to antibody fragment 1... |
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==CryoEM structure of modular PKS holo-Lsd14 bound to antibody fragment 1B2, composite structure== | |||
<StructureSection load='7s6d' size='340' side='right'caption='[[7s6d]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7s6d]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea] and [https://en.wikipedia.org/wiki/Streptomyces_lasalocidi Streptomyces lasalocidi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S6D FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATR:2-MONOPHOSPHOADENOSINE-5-DIPHOSPHATE'>ATR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s6d OCA], [https://pdbe.org/7s6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s6d RCSB], [https://www.ebi.ac.uk/pdbsum/7s6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s6d ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ERYA2_SACER ERYA2_SACER] [https://www.uniprot.org/uniprot/B6ZK67_STRLS B6ZK67_STRLS] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Type I modular polyketide synthases are homodimeric multidomain assembly line enzymes that synthesize a variety of polyketide natural products by performing polyketide chain extension and beta-keto group modification reactions. We determined the 2.4-angstrom-resolution x-ray crystal structure and the 3.1-angstrom-resolution cryo-electron microscopy structure of the Lsd14 polyketide synthase, stalled at the transacylation and condensation steps, respectively. These structures revealed how the constituent domains are positioned relative to each other, how they rearrange depending on the step in the reaction cycle, and the specific interactions formed between the domains. Like the evolutionarily related mammalian fatty acid synthase, Lsd14 contains two reaction chambers, but only one chamber in Lsd14 has the full complement of catalytic domains, indicating that only one chamber produces the polyketide product at any given time. | |||
Modular polyketide synthase contains two reaction chambers that operate asynchronously.,Bagde SR, Mathews II, Fromme JC, Kim CY Science. 2021 Nov 5;374(6568):723-729. doi: 10.1126/science.abi8532. Epub 2021 , Nov 4. PMID:34735234<ref>PMID:34735234</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Bagde | <div class="pdbe-citations 7s6d" style="background-color:#fffaf0;"></div> | ||
[[Category: Fromme | |||
[[Category: Kim | ==See Also== | ||
*[[Antibody 3D structures|Antibody 3D structures]] | |||
*[[6-deoxyerythronolide B synthase 3D structures|6-deoxyerythronolide B synthase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Saccharopolyspora erythraea]] | |||
[[Category: Streptomyces lasalocidi]] | |||
[[Category: Bagde SR]] | |||
[[Category: Fromme JC]] | |||
[[Category: Kim C-Y]] | |||
Latest revision as of 09:45, 21 November 2024
CryoEM structure of modular PKS holo-Lsd14 bound to antibody fragment 1B2, composite structureCryoEM structure of modular PKS holo-Lsd14 bound to antibody fragment 1B2, composite structure
Structural highlights
FunctionPublication Abstract from PubMedType I modular polyketide synthases are homodimeric multidomain assembly line enzymes that synthesize a variety of polyketide natural products by performing polyketide chain extension and beta-keto group modification reactions. We determined the 2.4-angstrom-resolution x-ray crystal structure and the 3.1-angstrom-resolution cryo-electron microscopy structure of the Lsd14 polyketide synthase, stalled at the transacylation and condensation steps, respectively. These structures revealed how the constituent domains are positioned relative to each other, how they rearrange depending on the step in the reaction cycle, and the specific interactions formed between the domains. Like the evolutionarily related mammalian fatty acid synthase, Lsd14 contains two reaction chambers, but only one chamber in Lsd14 has the full complement of catalytic domains, indicating that only one chamber produces the polyketide product at any given time. Modular polyketide synthase contains two reaction chambers that operate asynchronously.,Bagde SR, Mathews II, Fromme JC, Kim CY Science. 2021 Nov 5;374(6568):723-729. doi: 10.1126/science.abi8532. Epub 2021 , Nov 4. PMID:34735234[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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