7s4l: Difference between revisions

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-'''Unreleased structure'''
-The entry 7s4l is ON HOLD
+==CryoEM structure of Methylotuvimicrobium alcaliphilum 20Z pMMO in a POPC nanodisc at 2.46 Angstrom resolution==
+<StructureSection load='7s4l' size='340' side='right'caption='[[7s4l]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7s4l]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylotuvimicrobium_alcaliphilum_20Z Methylotuvimicrobium alcaliphilum 20Z]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S4L FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.46&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6ER:(S)-2,3-bis(hexanoyloxy)propyl(2-(trimethylammonio)ethyl)phosphate'>6ER</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=HXG:1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>HXG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s4l OCA], [https://pdbe.org/7s4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s4l RCSB], [https://www.ebi.ac.uk/pdbsum/7s4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s4l ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/G4SZ64_META2 G4SZ64_META2]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function.
-Authors:
+Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer.,Koo CW, Tucci FJ, He Y, Rosenzweig AC Science. 2022 Mar 18;375(6586):1287-1291. doi: 10.1126/science.abm3282. Epub 2022 , Mar 17. PMID:35298269<ref>PMID:35298269</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7s4l" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Methane monooxygenase 3D structures|Methane monooxygenase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Methylotuvimicrobium alcaliphilum 20Z]]
+[[Category: Koo CW]]
+[[Category: Rosenzweig AC]]