7ryk: Difference between revisions
New page: '''Unreleased structure''' The entry 7ryk is ON HOLD Authors: Han, Q., Darmanin, C., Smith, K., Drummond, C., Greaves, T. Description: Hen egg-white lysozyme with ionic liquid ethanola... |
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The | ==Hen egg-white lysozyme with ionic liquid ethanolammonium nitrate 1 mol%== | ||
<StructureSection load='7ryk' size='340' side='right'caption='[[7ryk]], [[Resolution|resolution]] 1.76Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7ryk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RYK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RYK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ryk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ryk OCA], [https://pdbe.org/7ryk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ryk RCSB], [https://www.ebi.ac.uk/pdbsum/7ryk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ryk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ions are important to modulate protein properties, including solubility and stability, through specific ion effects. Ionic liquids (ILs) are designer salts with versatile ion combinations with great potential to control protein properties. Although protein-ion binding of common metals is well-known, the IL effect on proteins is not well understood. Here, we employ the model protein lysozyme in dilute and concentrated IL solutions to determine the specific ion binding effect on protein phase behaviour, activity, size and conformational change, aggregation and intermolecular interactions. A combination of spectroscopic techniques, activity assays, small-angle X-ray scattering, and crystallography highlights that ILs, particularly their anions, bind to specific sites in the protein hydration layer via polar contacts on charged, polar and aromatic residues. The specific ion binding can induce more flexible loop regions in lysozyme, while the ion binding in the bulk phase can be more dynamic in solution. Overall, the protein behaviour in ILs depends on the net effect of nonspecific interactions and specific ion binding. Compared to formate, the nitrate anion induced high protein solubility, low activity, elongated shape and aggregation, which is largely owing to its higher propensity for ion binding. These findings provide new insights into protein-IL binding interactions and using ILs to modulate protein properties. | |||
Probing ion-binding at a protein interface: Modulation of protein properties by ionic liquids.,Han Q, Su Y, Smith KM, Binns J, Drummond CJ, Darmanin C, Greaves TL J Colloid Interface Sci. 2023 Nov 15;650(Pt B):1393-1405. doi: , 10.1016/j.jcis.2023.07.045. Epub 2023 Jul 11. PMID:37480654<ref>PMID:37480654</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Darmanin | <div class="pdbe-citations 7ryk" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Gallus gallus]] | |||
[[Category: Large Structures]] | |||
[[Category: Darmanin C]] | |||
[[Category: Drummond C]] | |||
[[Category: Greaves T]] | |||
[[Category: Han Q]] | |||
[[Category: Smith K]] | |||