7f0r: Difference between revisions

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-'''Unreleased structure'''
-The entry 7f0r is ON HOLD  until 2023-07-06
+==Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex==
+<StructureSection load='7f0r' size='340' side='right'caption='[[7f0r]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7f0r]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F0R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f0r OCA], [https://pdbe.org/7f0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f0r RCSB], [https://www.ebi.ac.uk/pdbsum/7f0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f0r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPOA_PSEAE RPOA_PSEAE] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_00059]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Pseudomonas aeruginosa (Pae) SutA adapts bacteria to hypoxia and nutrition-limited environment during chronic infection by increasing transcription activity of an RNA polymerase (RNAP) holoenzyme comprising the stress-responsive sigma factor sigma(S) (RNAP-sigma(S)). SutA shows no homology to previously characterized RNAP-binding proteins. The structure and mode of action of SutA remain unclear. Here we determined cryo-EM structures of Pae RNAP-sigma(S) holoenzyme, Pae RNAP-sigma(S) holoenzyme complexed with SutA, and Pae RNAP-sigma(S) transcription initiation complex comprising SutA. The structures show SutA pinches RNAP-beta protrusion and facilitates promoter unwinding by wedging RNAP-beta lobe open. Our results demonstrate that SutA clears an energetic barrier to facilitate promoter unwinding of RNAP-sigma(S) holoenzyme.
-Authors:
+Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding.,He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y Nat Commun. 2022 Jul 20;13(1):4204. doi: 10.1038/s41467-022-31871-7. PMID:35859063<ref>PMID:35859063</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7f0r" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
+*[[Sigma factor 3D structures|Sigma factor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Pseudomonas aeruginosa PAO1]]
+[[Category: Synthetic construct]]
+[[Category: He DW]]
+[[Category: You LL]]
+[[Category: Zhang Y]]