7eu0: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 7eu0 is ON HOLD
+==The cryo-EM structure of A. thaliana Pol IV-RDR2 backtracked complex==
+<StructureSection load='7eu0' size='340' side='right'caption='[[7eu0]], [[Resolution|resolution]] 3.16&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7eu0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EU0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.16&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eu0 OCA], [https://pdbe.org/7eu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eu0 RCSB], [https://www.ebi.ac.uk/pdbsum/7eu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eu0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NRPD1_ARATH NRPD1_ARATH] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity). Largest and catalytic component of RNA polymerase IV which mediates 24-nt short-interfering RNAs (siRNA) accumulation. Implicated in siRNA-directed heterochromatin formation through the action of DCL3 and AGO4, and subsequent DNA methylation-dependent silencing of targeted sequences. Essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production. Required for intercellular but not intracellular RNA interference (RNAi) leading to systemic post-transcriptional gene silencing. Involved in the maintenance of post-transcriptional RNA silencing.<ref>PMID:10850496</ref> <ref>PMID:12198169</ref> <ref>PMID:15692015</ref> <ref>PMID:15766525</ref> <ref>PMID:16140984</ref> <ref>PMID:16273107</ref> <ref>PMID:16377568</ref> <ref>PMID:16839878</ref> <ref>PMID:17360559</ref> <ref>PMID:17526749</ref> <ref>PMID:17558406</ref> <ref>PMID:17785412</ref> <ref>PMID:18287047</ref> <ref>PMID:18433438</ref> <ref>PMID:18467467</ref> <ref>PMID:19110459</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+DNA methylation affects gene expression and maintains genome integrity. The DNA-dependent RNA polymerase IV (Pol IV), together with the RNA-dependent RNA polymerase RDR2, produces double-stranded small interfering RNA precursors essential for establishing and maintaining DNA methylation in plants. We determined the cryo-electron microscopy structures of the Pol IV-RDR2 holoenzyme and the backtracked transcription elongation complex. These structures reveal that Pol IV and RDR2 form a complex with their active sites connected by an interpolymerase channel, through which the Pol IV-generated transcript is handed over to the RDR2 active site after being backtracked, where it is used as the template for double-stranded RNA (dsRNA) synthesis. Our results describe a 'backtracking-triggered RNA channeling' mechanism underlying dsRNA synthesis and also shed light on the evolutionary trajectory of eukaryotic RNA polymerases.
-Authors: Huang, K., Wu, X.X., Fang, C.L., Zhang, Y.
+Pol IV and RDR2: A two-RNA-polymerase machine that produces double-stranded RNA.,Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y Science. 2021 Dec 24;374(6575):1579-1586. doi: 10.1126/science.abj9184. Epub 2021 , Dec 23. PMID:34941388<ref>PMID:34941388</ref>
-Description: The cryo-EM structure of A. thaliana Pol IV-RDR2 backtracked complex
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Huang, K]]
+<div class="pdbe-citations 7eu0" style="background-color:#fffaf0;"></div>
-[[Category: Zhang, Y]]
-[[Category: Wu, X.X]]
+==See Also==
-[[Category: Fang, C.L]]
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Fang CL]]
+[[Category: Huang K]]
+[[Category: Wu XX]]
+[[Category: Zhang Y]]