Lysine-cysteine NOS bonds: Difference between revisions
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==Prevalence== | ==Prevalence== | ||
A survey of the data in the [[Protein Data Bank]] revealed that the NOS bond likely exists "in diverse protein families across all domains of life (including ''Homo sapiens'') and that it is often located at catalytic or regulatory hotspots."<ref name="wensien2021" /> They found that about 100-150 or ~0.3% of entries in the PDB with resolutions of 2.0 Å or better have Lys-Cys NOS bonds<ref name=" | A survey of the data in the [[Protein Data Bank]] revealed that the NOS bond likely exists "in diverse protein families across all domains of life (including ''Homo sapiens'') and that it is often located at catalytic or regulatory hotspots."<ref name="wensien2021" /> They found that about 100-150 or ~0.3% of entries in the PDB with resolutions of 2.0 Å or better have Lys-Cys NOS bonds<ref name="prevalence">PMID: 35165445</ref>. Because the NOS bond was unknown before 2019, it was often overlooked in earlier interpretations of [[electron density maps]].<ref name="wensien2021" /> Three examples are given illustrating putative NOS bonds in active sites of enzymes which, if correct, were previously overlooked: | ||
*[[1m3q]] 2004, a DNA glycosylase: Lys249 – Cys253. | *[[1m3q]] 2004, a DNA glycosylase: Lys249 – Cys253. | ||
*[[5y72]] 2018, a prenyltransferase: Lys275 – Cys223. | *[[5y72]] 2018, a prenyltransferase: Lys275 – Cys223. | ||
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==Other Protein Crosslinks== | ==Other Protein Crosslinks== | ||
In addition to the | In addition to the bonds discussed above, other [[Protein crosslinks|covalent cross-links between polypeptide chains]] include: | ||
*[[Disulfide bond]]s | *[[Disulfide bond]]s | ||
*[[Isopeptide bond]]s | *[[Isopeptide bond]]s | ||