7ogk: Difference between revisions
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==A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation. PNPase-3'ETS(leuZ)== | |||
<StructureSection load='7ogk' size='340' side='right'caption='[[7ogk]], [[Resolution|resolution]] 3.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7ogk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OGK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ogk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ogk OCA], [https://pdbe.org/7ogk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ogk RCSB], [https://www.ebi.ac.uk/pdbsum/7ogk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ogk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PNP_ECOLI PNP_ECOLI] Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction.[HAMAP-Rule:MF_01595] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Polynucleotide phosphorylase (PNPase) is an ancient exoribonuclease conserved in the course of evolution and is found in species as diverse as bacteria and humans. Paradoxically, Escherichia coli PNPase can act not only as an RNA degrading enzyme but also by an unknown mechanism as a chaperone for small regulatory RNAs (sRNAs), with pleiotropic consequences for gene regulation. We present structures of the ternary assembly formed by PNPase, the RNA chaperone Hfq, and sRNA and show that this complex boosts sRNA stability in vitro. Comparison of structures for PNPase in RNA carrier and degradation modes reveals how the RNA is rerouted away from the active site through interactions with Hfq and the KH and S1 domains. Together, these data explain how PNPase is repurposed to protect sRNAs from cellular ribonucleases such as RNase E and could aid RNA presentation to facilitate regulatory actions on target genes. | |||
A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation.,Dendooven T, Sinha D, Roeselova A, Cameron TA, De Lay NR, Luisi BF, Bandyra KJ Mol Cell. 2021 Jun 17. pii: S1097-2765(21)00445-7. doi:, 10.1016/j.molcel.2021.05.032. PMID:34157309<ref>PMID:34157309</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7ogk" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] | ||
[[Category: Luisi | == References == | ||
[[Category: Roesoleva | <references/> | ||
[[Category: Sinha | __TOC__ | ||
</StructureSection> | |||
[[Category: Escherichia coli]] | |||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | |||
[[Category: Bandyra K]] | |||
[[Category: Cameron TA]] | |||
[[Category: De Lay N]] | |||
[[Category: Dendooven T]] | |||
[[Category: Luisi BF]] | |||
[[Category: Roesoleva A]] | |||
[[Category: Sinha D]] | |||