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==Structure and Activity of SLAC1 Channels for Stomatal Signaling in Leaves== | ==Structure and Activity of SLAC1 Channels for Stomatal Signaling in Leaves== | ||
<StructureSection load='7en0' size='340' side='right'caption='[[7en0]]' scene=''> | <StructureSection load='7en0' size='340' side='right'caption='[[7en0]], [[Resolution|resolution]] 2.97Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EN0 FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EN0 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7en0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7en0 OCA], [https://pdbe.org/7en0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7en0 RCSB], [https://www.ebi.ac.uk/pdbsum/7en0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7en0 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.97Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLC:DIUNDECYL+PHOSPHATIDYL+CHOLINE'>PLC</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7en0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7en0 OCA], [https://pdbe.org/7en0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7en0 RCSB], [https://www.ebi.ac.uk/pdbsum/7en0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7en0 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Stomata in leaves regulate gas exchange between the plant and its atmosphere. Various environmental stimuli elicit abscisic acid (ABA); ABA leads to phosphoactivation of slow anion channel 1 (SLAC1); SLAC1 activity reduces turgor pressure in aperture-defining guard cells; and stomatal closure ensues. We used electrophysiology for functional characterizations of Arabidopsis thaliana SLAC1 (AtSLAC1) and cryoelectron microscopy (cryo-EM) for structural analysis of Brachypodium distachyon SLAC1 (BdSLAC1), at 2.97-A resolution. We identified 14 phosphorylation sites in AtSLAC1 and showed nearly 330-fold channel-activity enhancement with 4 to 6 of these phosphorylated. Seven SLAC1-conserved arginines are poised in BdSLAC1 for regulatory interaction with the N-terminal extension. This BdSLAC1 structure has its pores closed, in a basal state, spring loaded by phenylalanyl residues in high-energy conformations. SLAC1 phosphorylation fine-tunes an equilibrium between basal and activated SLAC1 trimers, thereby controlling the degree of stomatal opening. | |||
Structure and activity of SLAC1 channels for stomatal signaling in leaves.,Deng YN, Kashtoh H, Wang Q, Zhen GX, Li QY, Tang LH, Gao HL, Zhang CR, Qin L, Su M, Li F, Huang XH, Wang YC, Xie Q, Clarke OB, Hendrickson WA, Chen YH Proc Natl Acad Sci U S A. 2021 May 4;118(18). pii: 2015151118. doi:, 10.1073/pnas.2015151118. PMID:33926963<ref>PMID:33926963</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7en0" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ion channels 3D structures|Ion channels 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||