7m6l: Difference between revisions
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==High resolution structure of the membrane embedded skeletal muscle ryanodine receptor== | |||
<StructureSection load='7m6l' size='340' side='right'caption='[[7m6l]], [[Resolution|resolution]] 3.98Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7m6l]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M6L FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.98Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CFF:CAFFEINE'>CFF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m6l OCA], [https://pdbe.org/7m6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m6l RCSB], [https://www.ebi.ac.uk/pdbsum/7m6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m6l ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RYR1_RABIT RYR1_RABIT] Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis (By similarity).<ref>PMID:10388749</ref> <ref>PMID:22036948</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The type 1 ryanodine receptor (RyR)/calcium release channel on the sarcoplasmic reticulum (SR) is required for skeletal muscle excitation-contraction coupling and is the largest known ion channel, composed of four 565-kDa protomers. Cryogenic electron microscopy (cryo-EM) studies of the RyR have primarily used detergent to solubilize the channel; in the present study, we have used cryo-EM to solve high-resolution structures of the channel in liposomes using a gel-filtration approach with on-column detergent removal to form liposomes and incorporate the channel simultaneously. This allowed us to resolve the structure of the channel in the primed and open states at 3.4 and 4.0 A, respectively, with a single dataset. This method offers validation for detergent-based structures of the RyR and offers a starting point for utilizing a chemical gradient mimicking the SR, where Ca(2+) concentrations are millimolar in the lumen and nanomolar in the cytosol. | |||
High-resolution structure of the membrane-embedded skeletal muscle ryanodine receptor.,Melville Z, Kim K, Clarke OB, Marks AR Structure. 2022 Jan 6;30(1):172-180.e3. doi: 10.1016/j.str.2021.08.001. Epub 2021 , Aug 31. PMID:34469755<ref>PMID:34469755</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7m6l" style="background-color:#fffaf0;"></div> | ||
[[Category: Kim | |||
[[Category: | ==See Also== | ||
[[Category: | *[[FKBP 3D structures|FKBP 3D structures]] | ||
*[[Ryanodine receptor 3D structures|Ryanodine receptor 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Oryctolagus cuniculus]] | |||
[[Category: Clarke OB]] | |||
[[Category: Kim K]] | |||
[[Category: Marks AR]] | |||
[[Category: Melville Z]] | |||