7nl0: Difference between revisions
New page: '''Unreleased structure''' The entry 7nl0 is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Cryo-EM structure of the Lin28B nucleosome core particle== | |||
<StructureSection load='7nl0' size='340' side='right'caption='[[7nl0]], [[Resolution|resolution]] 3.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7nl0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NL0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nl0 OCA], [https://pdbe.org/7nl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nl0 RCSB], [https://www.ebi.ac.uk/pdbsum/7nl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nl0 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/H31_HUMAN H31_HUMAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pioneer transcription factors such as OCT4 can target silent genes embedded in nucleosome-dense regions. How nucleosome interaction enables transcription factors to target chromatin and determine cell identity remains elusive. Here, we systematically dissect OCT4 to show that nucleosome binding is encoded within the DNA-binding domain and yet can be uncoupled from free-DNA binding. Furthermore, accelerating the binding kinetics of OCT4 to DNA enhances nucleosome binding. In cells, uncoupling nucleosome binding diminishes the ability of OCT4 to individually access closed chromatin, while more dynamic nucleosome binding results in expansive genome scanning within closed chromatin. However, both uncoupling and enhancing nucleosome binding are detrimental to inducing pluripotency from differentiated cells. Remarkably, stable interactions between OCT4 and nucleosomes are continuously required for maintaining the accessibility of pluripotency enhancers in stem cells. Our findings reveal how the affinity and residence time of OCT4-nucleosome complexes modulate chromatin accessibility during cell fate changes and maintenance. | |||
Dissecting OCT4 defines the role of nucleosome binding in pluripotency.,Roberts GA, Ozkan B, Gachulincova I, O'Dwyer MR, Hall-Ponsele E, Saxena M, Robinson PJ, Soufi A Nat Cell Biol. 2021 Aug;23(8):834-845. doi: 10.1038/s41556-021-00727-5. Epub 2021 , Aug 5. PMID:34354236<ref>PMID:34354236</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7nl0" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Histone 3D structures|Histone 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Gachulincova I]] | |||
[[Category: Hall-Ponsele E]] | |||
[[Category: O Dwyer MR]] | |||
[[Category: Ozkan B]] | |||
[[Category: Roberts GA]] | |||
[[Category: Robinson PJ]] | |||
[[Category: Saxena M]] | |||
[[Category: Soufi A]] | |||
Latest revision as of 11:57, 14 July 2024
Cryo-EM structure of the Lin28B nucleosome core particleCryo-EM structure of the Lin28B nucleosome core particle
Structural highlights
FunctionPublication Abstract from PubMedPioneer transcription factors such as OCT4 can target silent genes embedded in nucleosome-dense regions. How nucleosome interaction enables transcription factors to target chromatin and determine cell identity remains elusive. Here, we systematically dissect OCT4 to show that nucleosome binding is encoded within the DNA-binding domain and yet can be uncoupled from free-DNA binding. Furthermore, accelerating the binding kinetics of OCT4 to DNA enhances nucleosome binding. In cells, uncoupling nucleosome binding diminishes the ability of OCT4 to individually access closed chromatin, while more dynamic nucleosome binding results in expansive genome scanning within closed chromatin. However, both uncoupling and enhancing nucleosome binding are detrimental to inducing pluripotency from differentiated cells. Remarkably, stable interactions between OCT4 and nucleosomes are continuously required for maintaining the accessibility of pluripotency enhancers in stem cells. Our findings reveal how the affinity and residence time of OCT4-nucleosome complexes modulate chromatin accessibility during cell fate changes and maintenance. Dissecting OCT4 defines the role of nucleosome binding in pluripotency.,Roberts GA, Ozkan B, Gachulincova I, O'Dwyer MR, Hall-Ponsele E, Saxena M, Robinson PJ, Soufi A Nat Cell Biol. 2021 Aug;23(8):834-845. doi: 10.1038/s41556-021-00727-5. Epub 2021 , Aug 5. PMID:34354236[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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