7nd0: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Unreleased structure'''
-The entry 7nd0 is ON HOLD
+==lateral-open conformation of the wild-type BAM complex (BamABCDE) bound to a bactericidal Fab fragment==
+<StructureSection load='7nd0' size='340' side='right'caption='[[7nd0]], [[Resolution|resolution]] 5.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7nd0]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ND0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ND0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nd0 OCA], [https://pdbe.org/7nd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7nd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nd0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/BAMD_ECOLI BAMD_ECOLI] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamA, the core component of the assembly machinery. Probably involved in transient protein interactions.[HAMAP-Rule:MF_00922]<ref>PMID:16824102</ref> <ref>PMID:20378773</ref> <ref>PMID:21586578</ref> <ref>PMID:21823654</ref> <ref>PMID:22281737</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The folding of beta-barrel outer membrane proteins (OMPs) in Gram-negative bacteria is catalysed by the beta-barrel assembly machinery (BAM). How lateral opening in the beta-barrel of the major subunit BamA assists in OMP folding, and the contribution of membrane disruption to BAM catalysis remain unresolved. Here, we use an anti-BamA monoclonal antibody fragment (Fab1) and two disulphide-crosslinked BAM variants (lid-locked (LL), and POTRA-5-locked (P5L)) to dissect these roles. Despite being lethal in vivo, we show that all complexes catalyse folding in vitro, albeit less efficiently than wild-type BAM. CryoEM reveals that while Fab1 and BAM-P5L trap an open-barrel state, BAM-LL contains a mixture of closed and contorted, partially-open structures. Finally, all three complexes globally destabilise the lipid bilayer, while BamA does not, revealing that the BAM lipoproteins are required for this function. Together the results provide insights into the role of BAM structure and lipid dynamics in OMP folding.
-Authors:
+The role of membrane destabilisation and protein dynamics in BAM catalysed OMP folding.,White P, Haysom SF, Iadanza MG, Higgins AJ, Machin JM, Whitehouse JM, Horne JE, Schiffrin B, Carpenter-Platt C, Calabrese AN, Storek KM, Rutherford ST, Brockwell DJ, Ranson NA, Radford SE Nat Commun. 2021 Jul 7;12(1):4174. doi: 10.1038/s41467-021-24432-x. PMID:34234105<ref>PMID:34234105</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7nd0" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[Bam complex 3D structures|Bam complex 3D structures]]
+*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli K-12]]
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Haysom SH]]
+[[Category: Iadanza MG]]