7bin: Difference between revisions
New page: '''Unreleased structure''' The entry 7bin is ON HOLD Authors: Johnson, S., Furlong, E., Lea, S.M. Description: Salmonella export gate and rod refined in focussed C1 map [[Category: Unr... |
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The | ==Salmonella export gate and rod refined in focussed C1 map== | ||
<StructureSection load='7bin' size='340' side='right'caption='[[7bin]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7bin]] is a 56 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhi Salmonella enterica subsp. enterica serovar Typhi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bin OCA], [https://pdbe.org/7bin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bin RCSB], [https://www.ebi.ac.uk/pdbsum/7bin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bin ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/FLIP_SALTY FLIP_SALTY] Plays a role in the flagellum-specific transport system. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids(1-3). Here, we present structures of the intact Salmonella flagellar basal body(4), encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 A. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine. | |||
Molecular structure of the intact bacterial flagellar basal body.,Johnson S, Furlong EJ, Deme JC, Nord AL, Caesar JJE, Chevance FFV, Berry RM, Hughes KT, Lea SM Nat Microbiol. 2021 Apr 30. pii: 10.1038/s41564-021-00895-y. doi:, 10.1038/s41564-021-00895-y. PMID:33931760<ref>PMID:33931760</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Furlong | <div class="pdbe-citations 7bin" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Salmonella enterica subsp. enterica serovar Typhi]] | |||
[[Category: Furlong E]] | |||
[[Category: Johnson S]] | |||
[[Category: Lea SM]] | |||
Latest revision as of 11:55, 14 July 2024
Salmonella export gate and rod refined in focussed C1 mapSalmonella export gate and rod refined in focussed C1 map
Structural highlights
FunctionFLIP_SALTY Plays a role in the flagellum-specific transport system. Publication Abstract from PubMedThe bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids(1-3). Here, we present structures of the intact Salmonella flagellar basal body(4), encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 A. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine. Molecular structure of the intact bacterial flagellar basal body.,Johnson S, Furlong EJ, Deme JC, Nord AL, Caesar JJE, Chevance FFV, Berry RM, Hughes KT, Lea SM Nat Microbiol. 2021 Apr 30. pii: 10.1038/s41564-021-00895-y. doi:, 10.1038/s41564-021-00895-y. PMID:33931760[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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