7aio: Difference between revisions

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<StructureSection load='7aio' size='340' side='right'caption='[[7aio]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
<StructureSection load='7aio' size='340' side='right'caption='[[7aio]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7aio]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AIO FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AIO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.31&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7ain|7ain]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SLC12A6, KCC3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aio OCA], [https://pdbe.org/7aio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aio RCSB], [https://www.ebi.ac.uk/pdbsum/7aio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aio ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aio OCA], [https://pdbe.org/7aio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aio RCSB], [https://www.ebi.ac.uk/pdbsum/7aio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aio ProSAT]</span></td></tr>
</table>
</table>
== Disease ==
[[https://www.uniprot.org/uniprot/S12A6_HUMAN S12A6_HUMAN]] Corpus callosum agenesis-neuronopathy syndrome. The disease is caused by mutations affecting the gene represented in this entry.
== Function ==
[[https://www.uniprot.org/uniprot/S12A6_HUMAN S12A6_HUMAN]] Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Potassium-coupled chloride transporters (KCCs) play crucial roles in regulating cell volume and intracellular chloride concentration. They are characteristically inhibited under isotonic conditions via phospho-regulatory sites located within the cytoplasmic termini. Decreased inhibitory phosphorylation in response to hypotonic cell swelling stimulates transport activity, and dysfunction of this regulatory process has been associated with various human diseases. Here, we present cryo-EM structures of human KCC3b and KCC1, revealing structural determinants for phospho-regulation in both N- and C-termini. We show that phospho-mimetic KCC3b is arrested in an inward-facing state in which intracellular ion access is blocked by extensive contacts with the N-terminus. In another mutant with increased isotonic transport activity, KCC1Delta19, this interdomain interaction is absent, likely due to a unique phospho-regulatory site in the KCC1 N-terminus. Furthermore, we map additional phosphorylation sites as well as a previously unknown ATP/ADP-binding pocket in the large C-terminal domain and show enhanced thermal stabilization of other CCCs by adenine nucleotides. These findings provide fundamentally new insights into the complex regulation of KCCs and may unlock innovative strategies for drug development.


Phospho-regulation, nucleotide binding and ion access control in potassium-chloride cotransporters.,Chi G, Ebenhoch R, Man H, Tang H, Tremblay LE, Reggiano G, Qiu X, Bohstedt T, Liko I, Almeida FG, Garneau AP, Wang D, McKinley G, Moreau CP, Bountra KD, Abrusci P, Mukhopadhyay SMM, Fernandez-Cid A, Slimani S, Lavoie JL, Burgess-Brown NA, Tehan B, DiMaio F, Jazayeri A, Isenring P, Robinson CV, Durr KL EMBO J. 2021 May 25:e107294. doi: 10.15252/embj.2020107294. PMID:34031912<ref>PMID:34031912</ref>
==See Also==
 
*[[Solute carrier family 12 3D structures|Solute carrier family 12 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
*[[Symporter 3D structures|Symporter 3D structures]]
</div>
<div class="pdbe-citations 7aio" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Abrusci, P]]
[[Category: Abrusci P]]
[[Category: Arrowsmith, C H]]
[[Category: Arrowsmith CH]]
[[Category: Bohstedt, T]]
[[Category: Bohstedt T]]
[[Category: Bountra, C]]
[[Category: Bountra C]]
[[Category: Burgess-Brown, N A]]
[[Category: Burgess-Brown NA]]
[[Category: Chalk, R]]
[[Category: Chalk R]]
[[Category: Chi, G]]
[[Category: Chi G]]
[[Category: DiMaio, F]]
[[Category: DiMaio F]]
[[Category: Duerr, K L]]
[[Category: Duerr KL]]
[[Category: Ebenhoch, R]]
[[Category: Ebenhoch R]]
[[Category: Edwards, A M]]
[[Category: Edwards AM]]
[[Category: MacLean, E M]]
[[Category: MacLean EM]]
[[Category: Man, H]]
[[Category: Man H]]
[[Category: Marsden, B D]]
[[Category: Marsden BD]]
[[Category: McKinley, G]]
[[Category: McKinley G]]
[[Category: Moreau, C]]
[[Category: Moreau C]]
[[Category: Mukhopadhyay, S M.M]]
[[Category: Mukhopadhyay SMM]]
[[Category: Pike, A C.W]]
[[Category: Pike ACW]]
[[Category: Reggiano, G]]
[[Category: Reggiano G]]
[[Category: Structural genomic]]
[[Category: Singh NK]]
[[Category: Singh, N K]]
[[Category: Snee M]]
[[Category: Snee, M]]
[[Category: Wang D]]
[[Category: Wang, D]]
[[Category: Ccc]]
[[Category: Homodimer]]
[[Category: Human membrane protein]]
[[Category: Kcc]]
[[Category: Kcc3]]
[[Category: Potassium-chloride coupled transporter]]
[[Category: Sgc]]
[[Category: Transport protein]]
[[Category: Transporter]]

Latest revision as of 09:03, 21 November 2024

Structure of Human Potassium Chloride Transporter KCC3 S45D/T940D/T997D in NaCl (Subclass)Structure of Human Potassium Chloride Transporter KCC3 S45D/T940D/T997D in NaCl (Subclass)

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.31Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

7aio, resolution 3.31Å

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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