7jsx: Difference between revisions

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-'''Unreleased structure'''
-The entry 7jsx is ON HOLD  until Paper Publication
+==EPYC1(106-135) peptide-bound Rubisco==
+<StructureSection load='7jsx' size='340' side='right'caption='[[7jsx]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7jsx]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JSX FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsx OCA], [https://pdbe.org/7jsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsx RCSB], [https://www.ebi.ac.uk/pdbsum/7jsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RBL_CHLRE RBL_CHLRE] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_01338]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Approximately one-third of global CO(2) fixation occurs in a phase-separated algal organelle called the pyrenoid. The existing data suggest that the pyrenoid forms by the phase separation of the CO(2)-fixing enzyme Rubisco with a linker protein; however, the molecular interactions underlying this phase separation remain unknown. Here we present the structural basis of the interactions between Rubisco and its intrinsically disordered linker protein Essential Pyrenoid Component 1 (EPYC1) in the model alga Chlamydomonas reinhardtii. We find that EPYC1 consists of five evenly spaced Rubisco-binding regions that share sequence similarity. Single-particle cryo-electron microscopy of these regions in complex with Rubisco indicates that each Rubisco holoenzyme has eight binding sites for EPYC1, one on each Rubisco small subunit. Interface mutations disrupt binding, phase separation and pyrenoid formation. Cryo-electron tomography supports a model in which EPYC1 and Rubisco form a codependent multivalent network of specific low-affinity bonds, giving the matrix liquid-like properties. Our results advance the structural and functional understanding of the phase separation underlying the pyrenoid, an organelle that plays a fundamental role in the global carbon cycle.
-Authors: Matthies, D., He, S., Jonikas, M.C.
+The structural basis of Rubisco phase separation in the pyrenoid.,He S, Chou HT, Matthies D, Wunder T, Meyer MT, Atkinson N, Martinez-Sanchez A, Jeffrey PD, Port SA, Patena W, He G, Chen VK, Hughson FM, McCormick AJ, Mueller-Cajar O, Engel BD, Yu Z, Jonikas MC Nat Plants. 2020 Dec;6(12):1480-1490. doi: 10.1038/s41477-020-00811-y. Epub 2020 , Nov 23. PMID:33230314<ref>PMID:33230314</ref>
-Description: EPYC1(106-135) peptide-bound Rubisco
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Jonikas, M.C]]
+<div class="pdbe-citations 7jsx" style="background-color:#fffaf0;"></div>
-[[Category: He, S]]
-[[Category: Matthies, D]]
+==See Also==
+*[[RuBisCO 3D structures|RuBisCO 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Chlamydomonas reinhardtii]]
+[[Category: Large Structures]]
+[[Category: He S]]
+[[Category: Jonikas MC]]
+[[Category: Matthies D]]