7cn9: Difference between revisions
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The entry | ==Cryo-EM structure of SARS-CoV-2 Spike ectodomain== | ||
<StructureSection load='7cn9' size='340' side='right'caption='[[7cn9]], [[Resolution|resolution]] 4.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CN9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.7Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cn9 OCA], [https://pdbe.org/7cn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cn9 RCSB], [https://www.ebi.ac.uk/pdbsum/7cn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cn9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The influenza virus hemagglutinin (HA) and coronavirus spike (S) protein mediate virus entry. HA and S proteins are heavily glycosylated, making them potential targets for carbohydrate binding agents such as lectins. Here, we show that the lectin FRIL, isolated from hyacinth beans (Lablab purpureus), has anti-influenza and anti-SARS-CoV-2 activity. FRIL can neutralize 11 representative human and avian influenza strains at low nanomolar concentrations, and intranasal administration of FRIL is protective against lethal H1N1 infection in mice. FRIL binds preferentially to complex-type N-glycans and neutralizes viruses that possess complex-type N-glycans on their envelopes. As a homotetramer, FRIL is capable of aggregating influenza particles through multivalent binding and trapping influenza virions in cytoplasmic late endosomes, preventing their nuclear entry. Remarkably, FRIL also effectively neutralizes SARS-CoV-2, preventing viral protein production and cytopathic effect in host cells. These findings suggest a potential application of FRIL for the prevention and/or treatment of influenza and COVID-19. | |||
A Carbohydrate-Binding Protein from the Edible Lablab Beans Effectively Blocks the Infections of Influenza Viruses and SARS-CoV-2.,Liu YM, Shahed-Al-Mahmud M, Chen X, Chen TH, Liao KS, Lo JM, Wu YM, Ho MC, Wu CY, Wong CH, Jan JT, Ma C Cell Rep. 2020 Aug 11;32(6):108016. doi: 10.1016/j.celrep.2020.108016. Epub 2020 , Jul 24. PMID:32755598<ref>PMID:32755598</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7cn9" style="background-color:#fffaf0;"></div> | ||
[[Category: Chen | |||
[[Category: Ho | ==See Also== | ||
[[Category: Huang | *[[Spike protein 3D structures|Spike protein 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Wang | __TOC__ | ||
[[Category: Wu | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Chang Y]] | |||
[[Category: Chen T]] | |||
[[Category: Chen X]] | |||
[[Category: Ho M]] | |||
[[Category: Huang H]] | |||
[[Category: Lo JM]] | |||
[[Category: Ma C]] | |||
[[Category: Wang C]] | |||
[[Category: Wu Y]] | |||