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==Structure of apo telomerase from Candida Tropicalis== | ==Structure of apo telomerase from Candida Tropicalis== | ||
<StructureSection load='6zd6' size='340' side='right'caption='[[6zd6]]' scene=''> | <StructureSection load='6zd6' size='340' side='right'caption='[[6zd6]], [[Resolution|resolution]] 2.64Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZD6 FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZD6 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zd6 OCA], [https://pdbe.org/6zd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zd6 RCSB], [https://www.ebi.ac.uk/pdbsum/6zd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zd6 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.64Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zd6 OCA], [https://pdbe.org/6zd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zd6 RCSB], [https://www.ebi.ac.uk/pdbsum/6zd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zd6 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Telomerase plays critical roles in cellular aging, in the emergence and/or development of cancer, and in the capacity for stem-cell renewal, consists of a catalytic telomerase reverse transcriptase (TERT) and a template-encoding RNA (TER). TERs from diverse organisms contain two conserved structural elements: the template-pseudoknot (T-PK) and a helical three-way junction (TWJ). Species-specific features of the structure and function of telomerase make obtaining a more in-depth understanding of the molecular mechanism of telomerase particularly important. Here, we report the first structural studies of N-terminally truncated TERTs from Candida albicans and Candida tropicalis in apo form and complexed with their respective TWJs in several conformations. We found that Candida TERT proteins perform only one round of telomere addition in the presence or absence of PK/TWJ and display standard reverse transcriptase activity. The C-terminal domain adopts at least two extreme conformations and undergoes conformational interconversion, which regulates the catalytic activity. Most importantly, we identified a conserved tertiary structural motif, called the U-motif, which interacts with the reverse transcriptase domain and is crucial for catalytic activity. Together these results shed new light on the structure and mechanics of fungal TERTs, which show common TERT characteristics, but also display species-specific features. | |||
Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungidouble dagger.,Zhai LT, Rety S, Chen WF, Song ZY, Auguin D, Sun B, Dou SX, Xi XG Nucleic Acids Res. 2021 Apr 15. pii: 6226674. doi: 10.1093/nar/gkab261. PMID:33856462<ref>PMID:33856462</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6zd6" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Telomerase 3D structures|Telomerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||