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==Cryo-EM structure of zika virus complexed with Fab SIgN-3C at pH 8.0== | |||
<SX load='7bua' size='340' side='right' viewer='molstar' caption='[[7bua]], [[Resolution|resolution]] 4.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BUA FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bua OCA], [https://pdbe.org/7bua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bua RCSB], [https://www.ebi.ac.uk/pdbsum/7bua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bua ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Human antibody SIgN-3C neutralizes dengue virus (DENV) and Zika virus (ZIKV) differently. DENV:SIgN-3C Fab and ZIKV:SIgN-3C Fab cryoelectron microscopy (cryo-EM) complex structures show Fabs crosslink E protein dimers at extracellular pH 8.0 condition and also when further incubated at acidic endosomal conditions (pH 8.0-6.5). We observe Fab binding to DENV (pH 8.0-5.0) prevents virus fusion, and the number of bound Fabs increase (from 120 to 180). For ZIKV, although there are already 180 copies of Fab at pH 8.0, virus structural changes at pH 5.0 are not inhibited. The immunoglobulin G (IgG):DENV structure at pH 8.0 shows both Fab arms bind to epitopes around the 2-fold vertex. On ZIKV, an additional Fab around the 5-fold vertex at pH 8.0 suggests one IgG arm would engage with an epitope, although the other may bind to other viruses, causing aggregation. For DENV2 at pH 5.0, a similar scenario would occur, suggesting DENV2:IgG complex would aggregate in the endosome. Hence, a single antibody employs different neutralization mechanisms against different flaviviruses. | |||
A Human Antibody Neutralizes Different Flaviviruses by Using Different Mechanisms.,Zhang S, Loy T, Ng TS, Lim XN, Chew SV, Tan TY, Xu M, Kostyuchenko VA, Tukijan F, Shi J, Fink K, Lok SM Cell Rep. 2020 Apr 28;31(4):107584. doi: 10.1016/j.celrep.2020.107584. PMID:32348755<ref>PMID:32348755</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7bua" style="background-color:#fffaf0;"></div> | ||
[[Category: Kostyuchenko | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Antibody 3D structures|Antibody 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Chew SV]] | |||
[[Category: Kostyuchenko VA]] | |||
[[Category: Lim XN]] | |||
[[Category: Lok SM]] | |||
[[Category: Ng TS]] | |||
[[Category: Zhang S]] | |||
Latest revision as of 13:53, 23 October 2024
Cryo-EM structure of zika virus complexed with Fab SIgN-3C at pH 8.0Cryo-EM structure of zika virus complexed with Fab SIgN-3C at pH 8.0
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