6xu7: Difference between revisions
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==Drosophila melanogaster Testis polysome ribosome== | |||
<StructureSection load='6xu7' size='340' side='right'caption='[[6xu7]], [[Resolution|resolution]] 4.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6xu7]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XU7 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.9Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xu7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xu7 OCA], [https://pdbe.org/6xu7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xu7 RCSB], [https://www.ebi.ac.uk/pdbsum/6xu7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xu7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RSSA_DROME RSSA_DROME] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Required during oogenesis and imaginal development.<ref>PMID:7916731</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ribosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have characterised ribosomal protein heterogeneity across 4 tissues of Drosophila melanogaster. We find that testes and ovaries contain the most heterogeneous ribosome populations, which occurs through a combination of paralog-enrichment and paralog-switching. We have solved structures of ribosomes purified from in vivo tissues by cryo-EM, revealing differences in precise ribosomal arrangement for testis and ovary 80S ribosomes. Differences in the amino acid composition of paralog pairs and their localisation on the ribosome exterior indicate paralog-switching could alter the ribosome surface, enabling different proteins to regulate translation. One testis-specific paralog-switching pair is also found in humans, suggesting this is a conserved site of ribosome heterogeneity. Overall, this work allows us to propose that mRNA translation might be regulated in the gonads through ribosome heterogeneity, providing a potential means of ribosome specialisation. | |||
Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching.,Hopes T, Norris K, Agapiou M, McCarthy CGP, Lewis PA, O'Connell MJ, Fontana J, Aspden JL Nucleic Acids Res. 2022 Feb 28;50(4):2240-2257. doi: 10.1093/nar/gkab606. PMID:34283226<ref>PMID:34283226</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6xu7" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
[[Category: | *[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Drosophila melanogaster]] | |||
[[Category: Large Structures]] | |||
[[Category: Agapiou M]] | |||
[[Category: Aspden JL]] | |||
[[Category: Fontana J]] | |||
[[Category: Hopes T]] | |||
[[Category: McCarthy CGP]] | |||
[[Category: Norris K]] | |||
[[Category: OConnell MJ]] | |||
Latest revision as of 13:43, 23 October 2024
Drosophila melanogaster Testis polysome ribosomeDrosophila melanogaster Testis polysome ribosome
Structural highlights
FunctionRSSA_DROME Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Required during oogenesis and imaginal development.[1] Publication Abstract from PubMedRibosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have characterised ribosomal protein heterogeneity across 4 tissues of Drosophila melanogaster. We find that testes and ovaries contain the most heterogeneous ribosome populations, which occurs through a combination of paralog-enrichment and paralog-switching. We have solved structures of ribosomes purified from in vivo tissues by cryo-EM, revealing differences in precise ribosomal arrangement for testis and ovary 80S ribosomes. Differences in the amino acid composition of paralog pairs and their localisation on the ribosome exterior indicate paralog-switching could alter the ribosome surface, enabling different proteins to regulate translation. One testis-specific paralog-switching pair is also found in humans, suggesting this is a conserved site of ribosome heterogeneity. Overall, this work allows us to propose that mRNA translation might be regulated in the gonads through ribosome heterogeneity, providing a potential means of ribosome specialisation. Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching.,Hopes T, Norris K, Agapiou M, McCarthy CGP, Lewis PA, O'Connell MJ, Fontana J, Aspden JL Nucleic Acids Res. 2022 Feb 28;50(4):2240-2257. doi: 10.1093/nar/gkab606. PMID:34283226[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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