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==Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate== | |||
<SX load='6spf' size='340' side='right' viewer='molstar' caption='[[6spf]], [[Resolution|resolution]] 2.89Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6spf]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SPF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.89Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6spf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6spf OCA], [https://pdbe.org/6spf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6spf RCSB], [https://www.ebi.ac.uk/pdbsum/6spf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6spf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Resistance to antibiotics has become a major threat to modern medicine. The ribosome plays a fundamental role in cell vitality by the translation of the genetic code into proteins; hence, it is a major target for clinically useful antibiotics. We report here the cryo-electron microscopy structures of the ribosome of a pathogenic aminoglycoside (AG)-resistant Pseudomonas aeruginosa strain, as well as of a nonresistance strain isolated from a cystic fibrosis patient. The structural studies disclosed defective ribosome complex formation due to a conformational change of rRNA helix H69, an essential intersubunit bridge, and a secondary binding site of the AGs. In addition, a stable conformation of nucleotides A1486 and A1487, pointing into helix h44, is created compared to a non-AG-bound ribosome. We suggest that altering the conformations of ribosomal protein uL6 and rRNA helix H69, which interact with initiation-factor IF2, interferes with proper protein synthesis initiation. | |||
Structure of Pseudomonas aeruginosa ribosomes from an aminoglycoside-resistant clinical isolate.,Halfon Y, Jimenez-Fernandez A, La Rosa R, Espinosa Portero R, Krogh Johansen H, Matzov D, Eyal Z, Bashan A, Zimmerman E, Belousoff M, Molin S, Yonath A Proc Natl Acad Sci U S A. 2019 Oct 14. pii: 1909831116. doi:, 10.1073/pnas.1909831116. PMID:31611393<ref>PMID:31611393</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6spf" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
[[Category: | == References == | ||
[[Category: Halfon | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Krogh Johansen | </SX> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: | [[Category: Bashan A]] | ||
[[Category: | [[Category: Belousoff M]] | ||
[[Category: Espinos R]] | |||
[[Category: Eyal Z]] | |||
[[Category: Halfon Y]] | |||
[[Category: Jimenez-Fernande A]] | |||
[[Category: Krogh Johansen H]] | |||
[[Category: La Ros R]] | |||
[[Category: Matzov D]] | |||
[[Category: Molin S]] | |||
[[Category: Yonath A]] | |||
[[Category: Zimmerman E]] | |||