6joo: Difference between revisions

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 <StructureSection load='6joo' size='340' side='right'caption='[[6joo]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6joo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cordi Cordi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JOO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6joo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae] and [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae_NCTC_13129 Corynebacterium diphtheriae NCTC 13129]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JOO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cas9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=257309 CORDI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6joo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6joo OCA], [https://pdbe.org/6joo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6joo RCSB], [https://www.ebi.ac.uk/pdbsum/6joo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6joo ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6joo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6joo OCA], [http://pdbe.org/6joo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6joo RCSB], [http://www.ebi.ac.uk/pdbsum/6joo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6joo ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAS9_CORDI CAS9_CORDI] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA) (PubMed:25830891). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:25830891). PAM recognition is also required for catalytic activity (By similarity).[UniProtKB:Q99ZW2]<ref>PMID:25830891</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
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 </div>
 <div class="pdbe-citations 6joo" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Cordi]]
+[[Category: Corynebacterium diphtheriae]]
+[[Category: Corynebacterium diphtheriae NCTC 13129]]
 [[Category: Large Structures]]
-[[Category: Hirano, S]]
+[[Category: Hirano S]]
-[[Category: Ishitani, R]]
+[[Category: Ishitani R]]
-[[Category: Nishimasu, H]]
+[[Category: Nishimasu H]]
-[[Category: Nureki, O]]
+[[Category: Nureki O]]
-[[Category: Cas9]]
-[[Category: Crispr-ca]]
-[[Category: Dna endonuclease]]
-[[Category: Hydrolase-dna-rna complex]]
-[[Category: Pam]]
-[[Category: Ribonucleoprotein]]