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==Horse liver alcohol dehydrogenase determined using single-particle cryo-EM at 200 keV== | ==Horse liver alcohol dehydrogenase determined using single-particle cryo-EM at 200 keV== | ||
< | <SX load='6nbb' size='340' side='right' viewer='molstar' caption='[[6nbb]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6nbb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBB OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6nbb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NBB FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9Å, 10 models</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbb OCA], [https://pdbe.org/6nbb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nbb RCSB], [https://www.ebi.ac.uk/pdbsum/6nbb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/ADH1E_HORSE ADH1E_HORSE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Determining high-resolution structures of biological macromolecules amassing less than 100 kilodaltons (kDa) has been a longstanding goal of the cryo-electron microscopy (cryo-EM) community. While the Volta phase plate has enabled visualization of specimens in this size range, this instrumentation is not yet fully automated and can present technical challenges. Here, we show that conventional defocus-based cryo-EM methodologies can be used to determine high-resolution structures of specimens amassing less than 100 kDa using a transmission electron microscope operating at 200 keV coupled with a direct electron detector. Our ~2.7 A structure of alcohol dehydrogenase (82 kDa) proves that bound ligands can be resolved with high fidelity to enable investigation of drug-target interactions. Our ~2.8 A and ~3.2 A structures of methemoglobin demonstrate that distinct conformational states can be identified within a dataset for proteins as small as 64 kDa. Furthermore, we provide the sub-nanometer cryo-EM structure of a sub-50 kDa protein. | |||
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM.,Herzik MA Jr, Wu M, Lander GC Nat Commun. 2019 Mar 4;10(1):1032. doi: 10.1038/s41467-019-08991-8. PMID:30833564<ref>PMID:30833564</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6nbb" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</ | </SX> | ||
[[Category: | [[Category: Equus caballus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Herzik | [[Category: Herzik Jr MA]] | ||
[[Category: Lander | [[Category: Lander GC]] | ||
[[Category: Wu | [[Category: Wu M]] | ||
Latest revision as of 14:13, 30 October 2024
Horse liver alcohol dehydrogenase determined using single-particle cryo-EM at 200 keVHorse liver alcohol dehydrogenase determined using single-particle cryo-EM at 200 keV
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