6e15: Difference between revisions

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-'''Unreleased structure'''
-The entry 6e15 is ON HOLD
+==Handover mechanism of the growing pilus by the bacterial outer membrane usher FimD==
+<SX load='6e15' size='340' side='right' viewer='molstar' caption='[[6e15]], [[Resolution|resolution]] 5.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6e15]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E15 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e15 OCA], [https://pdbe.org/6e15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e15 RCSB], [https://www.ebi.ac.uk/pdbsum/6e15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e15 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FIMC_ECOLI FIMC_ECOLI] Required for the biogenesis of type 1 fimbriae. Binds and interact with FimH.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Pathogenic bacteria such as Escherichia coli assemble surface structures termed pili, or fimbriae, to mediate binding to host-cell receptors(1). Type 1 pili are assembled via the conserved chaperone-usher pathway(2-5). The outer-membrane usher FimD recruits pilus subunits bound by the chaperone FimC via the periplasmic N-terminal domain of the usher. Subunit translocation through the beta-barrel channel of the usher occurs at the two C-terminal domains (which we label CTD1 and CTD2) of this protein. How the chaperone-subunit complex bound to the N-terminal domain is handed over to the C-terminal domains, as well as the timing of subunit polymerization into the growing pilus, have previously been unclear. Here we use cryo-electron microscopy to capture a pilus assembly intermediate (FimD-FimC-FimF-FimG-FimH) in a conformation in which FimD is in the process of handing over the chaperone-bound end of the growing pilus to the C-terminal domains. In this structure, FimF has already polymerized with FimG, and the N-terminal domain of FimD swings over to bind CTD2; the N-terminal domain maintains contact with FimC-FimF, while at the same time permitting access to the C-terminal domains. FimD has an intrinsically disordered N-terminal tail that precedes the N-terminal domain. This N-terminal tail folds into a helical motif upon recruiting the FimC-subunit complex, but reorganizes into a loop to bind CTD2 during handover. Because both the N-terminal and C-terminal domains of FimD are bound to the end of the growing pilus, the structure further suggests a mechanism for stabilizing the assembly intermediate to prevent the pilus fibre diffusing away during the incorporation of thousands of subunits.
-Authors: Du, M., Yuan, Z., Yu, H., Henderson, N., Sarowar, S., Zhao, G., Werneburg, G.T., Thanassi, D.G., Li, H.
+Handover mechanism of the growing pilus by the bacterial outer-membrane usher FimD.,Du M, Yuan Z, Yu H, Henderson N, Sarowar S, Zhao G, Werneburg GT, Thanassi DG, Li H Nature. 2018 Oct 3. pii: 10.1038/s41586-018-0587-z. doi:, 10.1038/s41586-018-0587-z. PMID:30283140<ref>PMID:30283140</ref>
-Description: The N-terminus to C-terminus handover of chaperone-subunit by the pilus assembly usher
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Li, H]]
+<div class="pdbe-citations 6e15" style="background-color:#fffaf0;"></div>
-[[Category: Yu, H]]
-[[Category: Du, M]]
+==See Also==
-[[Category: Werneburg, G.T]]
+*[[Adhesin 3D structures|Adhesin 3D structures]]
-[[Category: Yuan, Z]]
+== References ==
-[[Category: Sarowar, S]]
+<references/>
-[[Category: Zhao, G]]
+__TOC__
-[[Category: Henderson, N]]
+</SX>
-[[Category: Thanassi, D.G]]
+[[Category: Escherichia coli]]
+[[Category: Large Structures]]
+[[Category: Du M]]
+[[Category: Henderson N]]
+[[Category: Li H]]
+[[Category: Sarowar S]]
+[[Category: Thanassi DG]]
+[[Category: Werneburg GT]]
+[[Category: Yu H]]
+[[Category: Yuan Z]]
+[[Category: Zhao G]]