6gtw: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 6gtw is ON HOLD
+==Crystal structure of the FimH lectin domain from E.coli F18 in complex with trimannose==
+<StructureSection load='6gtw' size='340' side='right'caption='[[6gtw]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6gtw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_F18+ Escherichia coli F18+]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GTW FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gtw OCA], [https://pdbe.org/6gtw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gtw RCSB], [https://www.ebi.ac.uk/pdbsum/6gtw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gtw ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Multivalent carbohydrate-lectin interactions at host-pathogen interfaces play a crucial role in the establishment of infections. Although competitive antagonists that prevent pathogen adhesion are promising anti-microbial drugs, the molecular mechanisms underlying these complex adhesion processes are still poorly understood. Here, we characterize the interactions between the fimbrial adhesin FimH from uropathogenic Escherichia coli strains and its natural high-mannose type N-glycan binding epitopes on uroepithelial glycoproteins. Crystal structures and a detailed kinetic characterization of ligand-binding and dissociation revealed that the binding pocket of FimH evolved such that it recognizes the terminal alpha(1-2)-, alpha(1-3)- and alpha(1-6)-linked mannosides of natural high-mannose type N-glycans with similar affinity. We demonstrate that the 2,000-fold higher affinity of the domain-separated state of FimH compared to its domain-associated state is ligand-independent and consistent with a thermodynamic cycle in which ligand-binding shifts the association equilibrium between the FimH lectin and the FimH pilin domain. Moreover, we show that a single N-glycan can bind up to three molecules of FimH, albeit with negative cooperativity, so that a molar excess of accessible N-glycans over FimH on the cell surface favors monovalent FimH binding. Our data provide pivotal insights into the adhesion properties of uropathogenic Escherichia coli strains to their target receptors and a solid basis for the development of effective FimH antagonists.
-Authors:
+Binding of the bacterial adhesin FimH to its natural, multivalent high-mannose type glycan targets.,Sauer MM, Jakob RP, Luber T, Canonica F, Navarra G, Ernst B, Unverzagt C, Maier T, Glockshuber R J Am Chem Soc. 2018 Dec 13. doi: 10.1021/jacs.8b10736. PMID:30543411<ref>PMID:30543411</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6gtw" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Adhesin 3D structures|Adhesin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli F18+]]
+[[Category: Large Structures]]
+[[Category: Canonica F]]
+[[Category: Ernst B]]
+[[Category: Glockshuber R]]
+[[Category: Jakob RP]]
+[[Category: Luber T]]
+[[Category: Maier T]]
+[[Category: Navarra G]]
+[[Category: Sauer MM]]
+[[Category: Unverzagt C]]