6fhv: Difference between revisions
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New page: '''Unreleased structure''' The entry 6fhv is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Crystal structure of Penicillium oxalicum Glucoamylase== | |||
<StructureSection load='6fhv' size='340' side='right'caption='[[6fhv]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6fhv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_oxalicum_114-2 Penicillium oxalicum 114-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FHV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhv OCA], [https://pdbe.org/6fhv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fhv RCSB], [https://www.ebi.ac.uk/pdbsum/6fhv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhv ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/S7ZIW0_PENO1 S7ZIW0_PENO1] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Glucoamylases are one of the most important classes of enzymes in the industrial degradation of starch biomass. They consist of a catalytic domain and a carbohydrate-binding domain (CBM), with the latter being important for the interaction with the polymeric substrate. Whereas the catalytic mechanisms and structures of the individual domains are well known, the spatial arrangement of the domains with respect to each other and its influence on activity are not fully understood. Here, the structures of three industrially used fungal glucoamylases, two of which are full length, have been crystallized and determined. It is shown for the first time that the relative orientation between the CBM and the catalytic domain is flexible, as they can adopt different orientations independently of ligand binding, suggesting a role as an anchor to increase the contact time and the relative concentration of substrate near the active site. The flexibility in the orientations of the two domains presented a considerable challenge for the crystallization of the enzymes. | |||
Structural insight into industrially relevant glucoamylases: flexible positions of starch-binding domains.,Roth C, Moroz OV, Ariza A, Skov LK, Ayabe K, Davies GJ, Wilson KS Acta Crystallogr D Struct Biol. 2018 May 1;74(Pt 5):463-470. doi:, 10.1107/S2059798318004989. Epub 2018 Apr 26. PMID:29717717<ref>PMID:29717717</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6fhv" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Penicillium oxalicum 114-2]] | |||
[[Category: Ariza A]] | |||
[[Category: Davies GJ]] | |||
[[Category: Friis EP]] | |||
[[Category: Moroz OV]] | |||
[[Category: Roth C]] | |||
[[Category: Wilson KS]] | |||