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| ==Cryo-EM structure of the core Centromere Binding Factor 3 complex== | | ==Cryo-EM structure of the core Centromere Binding Factor 3 complex== |
| <StructureSection load='6fe8' size='340' side='right'caption='[[6fe8]], [[Resolution|resolution]] 3.70Å' scene=''> | | <SX load='6fe8' size='340' side='right' viewer='molstar' caption='[[6fe8]], [[Resolution|resolution]] 4.10Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6fe8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FE8 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6fe8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FE8 FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6gsa|6gsa]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CEP3, CBF3, CBF3B, CSL1, YMR168C, YM8520.17C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SKP1, CBF3D, YDR328C, D9798.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), CTF13, CBF3C, YMR094W, YM6543.01, YM9582.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fe8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fe8 OCA], [https://pdbe.org/6fe8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fe8 RCSB], [https://www.ebi.ac.uk/pdbsum/6fe8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fe8 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fe8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fe8 OCA], [http://pdbe.org/6fe8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fe8 RCSB], [http://www.ebi.ac.uk/pdbsum/6fe8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fe8 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/CBF3B_YEAST CBF3B_YEAST]] Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. [[http://www.uniprot.org/uniprot/CBF3C_YEAST CBF3C_YEAST]] Acts as central component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. [[http://www.uniprot.org/uniprot/SKP1_YEAST SKP1_YEAST]] Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins like phosphorylated SIC1. Participates in the attachment of chromosomes to the spindle. Acts as a regulatory component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromeres and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. SKP1/CBF3D could retrieve cyclins or cyclin-CDK-like proteins into the kinetochore thus providing cell cycle-regulated kinetochore activity. Involved in the regulation of methionine biosynthesis genes. Facilitates association of CDC53 with CDC4 and of ROY1 with YPT52.<ref>PMID:8706132</ref> <ref>PMID:8706131</ref> <ref>PMID:9346238</ref> <ref>PMID:9346239</ref> <ref>PMID:21389113</ref> <ref>PMID:9499404</ref> <ref>PMID:17517885</ref> | | [https://www.uniprot.org/uniprot/CBF3B_YEAST CBF3B_YEAST] Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| </div> | | </div> |
| <div class="pdbe-citations 6fe8" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6fe8" style="background-color:#fffaf0;"></div> |
| | |
| | ==See Also== |
| | *[[Centromere protein 3D structure|Centromere protein 3D structure]] |
| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </SX> |
| [[Category: Atcc 18824]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Lukoynova, N]] | | [[Category: Saccharomyces cerevisiae]] |
| [[Category: Miah, S]] | | [[Category: Lukoynova N]] |
| [[Category: Vaughan, C K]] | | [[Category: Miah S]] |
| [[Category: Zhang, W J]] | | [[Category: Vaughan CK]] |
| [[Category: Cdeiii-binding]] | | [[Category: Zhang WJ]] |
| [[Category: Centromere]]
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| [[Category: Dna binding protein]]
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| [[Category: Lrr domain]]
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