Terminase: Difference between revisions

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<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>
<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>


 
__TOC__
== Function ==
== Function ==


'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses.  The Ter complex is comprised of a small Ter subunit which is a recognition subunit and a large Ter subunit which is an endonuclease/translocase subunit <ref>PMID:22297528</ref>.  The nuclease activity of the large Ter subunit is stimulated by ATP.
'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses.  The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>.  The nuclease activity of the large Ter subunit is stimulated by ATP.  The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits ('''TRM1, TRM2 and TRM3'''), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>.  '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.


== Structural highlights ==
== Structural highlights ==


The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
== 3D Structures of terminase ==
[[Terminase 3D Structures ]]
</StructureSection>
</StructureSection>
== 3D Structures of terminase ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
*Terminase small subunit
**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
**[[6ey7]] – HvTer nuclease domain + inhibitor<br />
**[[3hef]] – Ter – Bacteriophage T7 <br />
**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />
**[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br />
**[[3zqp]], [[3zqm]], [[3zqn]], [[3zqo]] – Sf6Ter oligomerization domain  <br />
**[[3zqq]], [[4dyq]], [[4dyr]] – Sf6Ter  <br />
**[[1j9i]] – Ter nuclease domain – Bacteriophage λ - NMR <br />
**[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br />
**[[3p9a]] – Ter – Bacteriophage P22 <br />
**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />
**[[6ejq]] – Ter – Thermus phage<br />
**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ  <br />
**[[7joq]] – Ter – Pseudomonas  phage NV1<br />
**[[6w7t]] – Ter – Pseudomonas  phage PaP3<br />
*Terminase large subunit
**[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br />
**[[4bij]], [[4bil]] – T7Ter – Bacteriophage T7 – Cryo EM<br />
**[[4idh]] – T7Ter <br />
**[[2wbn]], [[2wc9]] – Ter nuclease domain – Bacteriophage Spp1 <br />
**[[3c6a]], [[3c6h]] – Ter nuclease domain – Bacteriophage Rb49 <br />
**[[5c10]], [[5c12]] – Sf6Ter nuclease domain <br />
**[[5c2d]], [[5c15]] – Sf6Ter nuclease domain (mutant)<br />
**[[5m1f]], [[5m1k]], [[5m1n]], [[5m1o]], [[5m1p]], [[5m1q]] – G20cTer  <br />
**[[5oe8]], [[5oee]], [[5oe9]] – D6eTer – Bacteriophage D6e <br />
**[[5tge]] – P74Ter nuclease domain – Thermus phage P74 <br />
**[[4zni]], [[4znk]] – P74Ter ATPase domain  <br />
**[[4znj]] – P74Ter ATPase domain (mutant)<br />
**[[4znl]] – P74Ter ATPase domain + ADP <br />
*Terminase large subunit complex
**[[4iee]], [[4ife]] – Sf6Ter + ATP<br />
**[[4iei]] – Sf6Ter + ADP<br />
**[[5c2f]] – Sf6Ter nuclease domain (mutant) + antifungal drug<br />
**[[5oea]] – D6eTer + ATP <br />
**[[5oeb]] – D6eTer + ADP <br />
**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />
*Tripartite terminase
**[[6m5s]], [[6m5r]] – HvTer TRM1+TRM2+TRM3 – Cryo EM<br />
**[[6m5u]], [[6m5v]] – HvTer TRM1+TRM2+TRM3 + ADP – Cryo EM<br />
**[[6m5t]] – HvTer TRM3 – Cryo EM<br />
}}


== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 10:52, 18 August 2024


Function

Terminase (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a small Ter subunit which is a recognition subunit and a large Ter subunit which is an endonuclease/translocase subunit [1]. The nuclease activity of the large Ter subunit is stimulated by ATP. The tripartite terminase complex of herpesvirus which contains 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus[2]. TRM3 has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome[3].

Structural highlights

The large subunit of Ter is composed of an . ATP binds to the protein in a [4].

3D Structures of terminase

Terminase 3D Structures

Terminase large subunit complex with ATP (PDB code 4ife)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ PMID:22297528
  2. ↑ Sankhala RS, Lokareddy RK, Cingolani G. Divergent Evolution of Nuclear Localization Signal Sequences in Herpesvirus Terminase Subunits. J Biol Chem. 2016 Mar 31. pii: jbc.M116.724393. PMID:27033706 doi:http://dx.doi.org/10.1074/jbc.M116.724393
  3. ↑ Heming JD, Huffman JB, Jones LM, Homa FL. Isolation and characterization of the herpes simplex virus 1 terminase complex. J Virol. 2014 Jan;88(1):225-36. doi: 10.1128/JVI.02632-13. Epub 2013 Oct 23. PMID:24155374 doi:http://dx.doi.org/10.1128/JVI.02632-13
  4. ↑ Zhao H, Christensen TE, Kamau YN, Tang L. Structures of the phage Sf6 large terminase provide new insights into DNA translocation and cleavage. Proc Natl Acad Sci U S A. 2013 May 14;110(20):8075-8080. Epub 2013 Apr 29. PMID:23630261 doi:10.1073/pnas.1301133110

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