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==Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit== | ==Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit== | ||
< | <SX load='5t5h' size='340' side='right' viewer='molstar' caption='[[5t5h]], [[Resolution|resolution]] 2.54Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5t5h]] is a | <table><tr><td colspan='2'>[[5t5h]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T5H FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.54Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=A2M:2-O-METHYLADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>A2M</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5h OCA], [https://pdbe.org/5t5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t5h RCSB], [https://www.ebi.ac.uk/pdbsum/5t5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/RL18_TRYCC RL18_TRYCC] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA expansion segments, and r-protein variations compared with other eukaryotic ribosomes. To investigate the architecture of the trypanosomatid ribosomes, we determined the 2.5-A structure of the Trypanosoma cruzi ribosome large subunit by single-particle cryo-EM. Examination of this structure and comparative analysis of the yeast ribosomal assembly pathway allowed us to develop a stepwise assembly model for the eight pieces of the large subunit rRNAs and a number of ancillary "glue" proteins. This model can be applied to the characterization of Trypanosoma brucei and Leishmania spp. ribosomes as well. Together with other details, our atomic-level structure may provide a foundation for structure-based design of antitrypanosome drugs. | |||
Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.,Liu Z, Gutierrez-Vargas C, Wei J, Grassucci RA, Ramesh M, Espina N, Sun M, Tutuncuoglu B, Madison-Antenucci S, Woolford JL Jr, Tong L, Frank J Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12174-12179. Epub 2016 Oct 10. PMID:27791004<ref>PMID:27791004</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5t5h" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</ | </SX> | ||
[[Category: Large Structures]] | |||
[[Category: Trypanosoma cruzi]] | [[Category: Trypanosoma cruzi]] | ||
[[Category: Espina | [[Category: Espina N]] | ||
[[Category: Frank | [[Category: Frank J]] | ||
[[Category: Grassucci | [[Category: Grassucci RA]] | ||
[[Category: Gutierrez-Vargas | [[Category: Gutierrez-Vargas C]] | ||
[[Category: Liu | [[Category: Liu Z]] | ||
[[Category: Madison-Antenucci | [[Category: Madison-Antenucci S]] | ||
[[Category: Ramesh | [[Category: Ramesh M]] | ||
[[Category: Sun | [[Category: Sun M]] | ||
[[Category: Tong | [[Category: Tong L]] | ||
[[Category: Tutuncuoglu | [[Category: Tutuncuoglu B]] | ||
[[Category: Wei | [[Category: Wei J]] | ||
[[Category: Woolford | [[Category: Woolford Jr JL]] | ||
Latest revision as of 12:52, 25 December 2024
Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunitStructure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit
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