5t0c: Difference between revisions
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New page: '''Unreleased structure''' The entry 5t0c is ON HOLD Authors: Chen, S., Wu, J., Lu, Y., Ma, Y.B., Lee, B.H., Yu, Z., Ouyang, Q., Finley, D., Kirschner, M.W., Mao, Y. Description: Struc... |
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The | ==Structural basis for dynamic regulation of the human 26S proteasome== | ||
<SX load='5t0c' size='340' side='right' viewer='molstar' caption='[[5t0c]], [[Resolution|resolution]] 3.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5t0c]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T0C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t0c OCA], [https://pdbe.org/5t0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t0c RCSB], [https://www.ebi.ac.uk/pdbsum/5t0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t0c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PSMD1_HUMAN PSMD1_HUMAN] Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The proteasome is the major engine of protein degradation in all eukaryotic cells. At the heart of this machine is a heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitylated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides. Using cryoelectron microscopy, we determined a near-atomic-resolution structure of the 2.5-MDa human proteasome in its ground state, as well as subnanometer-resolution structures of the holoenzyme in three alternative conformational states. The substrate-unfolding AAA-ATPase channel is narrowed by 10 inward-facing pore loops arranged into two helices that run in parallel with each other, one hydrophobic in character and the other highly charged. The gate of the core particle was unexpectedly found closed in the ground state and open in only one of the alternative states. Coordinated, stepwise conformational changes of the regulatory particle couple ATP hydrolysis to substrate translocation and regulate gating of the core particle, leading to processive degradation. | |||
Structural basis for dynamic regulation of the human 26S proteasome.,Chen S, Wu J, Lu Y, Ma YB, Lee BH, Yu Z, Ouyang Q, Finley DJ, Kirschner MW, Mao Y Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):12991-12996. Epub 2016 Oct 21. PMID:27791164<ref>PMID:27791164</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5t0c" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Proteasome 3D structures|Proteasome 3D structures]] | ||
[[Category: | == References == | ||
[[Category: Ma | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </SX> | ||
[[Category: Wu | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Chen S]] | |||
[[Category: Finley D]] | |||
[[Category: Kirschner MW]] | |||
[[Category: Lee BH]] | |||
[[Category: Lu Y]] | |||
[[Category: Ma YB]] | |||
[[Category: Mao Y]] | |||
[[Category: Ouyang Q]] | |||
[[Category: Wu J]] | |||
[[Category: Yu Z]] | |||
Latest revision as of 15:11, 6 November 2024
Structural basis for dynamic regulation of the human 26S proteasomeStructural basis for dynamic regulation of the human 26S proteasome
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