5lme: Difference between revisions
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==Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase== | ==Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase== | ||
<StructureSection load='5lme' size='340' side='right'caption='[[5lme | <StructureSection load='5lme' size='340' side='right'caption='[[5lme]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5lme]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LME OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5lme]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoplusia_ni Trichoplusia ni]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LME FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lme OCA], [https://pdbe.org/5lme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lme RCSB], [https://www.ebi.ac.uk/pdbsum/5lme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lme ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q27026_TRINI Q27026_TRINI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5lme" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5lme" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Transposase 3D structures|Transposase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bardiaux | [[Category: Trichoplusia ni]] | ||
[[Category: Betermier | [[Category: Bardiaux B]] | ||
[[Category: Bischerour | [[Category: Betermier M]] | ||
[[Category: Craig | [[Category: Bischerour J]] | ||
[[Category: Dyda | [[Category: Craig NL]] | ||
[[Category: Guittet | [[Category: Dyda F]] | ||
[[Category: Hickman | [[Category: Guittet E]] | ||
[[Category: Lescop | [[Category: Hickman AB]] | ||
[[Category: Li | [[Category: Lescop E]] | ||
[[Category: Mathy | [[Category: Li X]] | ||
[[Category: Morellet | [[Category: Mathy N]] | ||
[[Category: Moriau | [[Category: Morellet N]] | ||
[[Category: Nilges | [[Category: Moriau S]] | ||
[[Category: Taylor | [[Category: Nilges M]] | ||
[[Category: Wieninger | [[Category: Taylor JA]] | ||
[[Category: Wieninger S]] | |||
Latest revision as of 09:02, 19 June 2024
Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposaseSpecific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase
Structural highlights
FunctionPublication Abstract from PubMedThe piggyBac transposase (PB) is distinguished by its activity and utility in genome engineering, especially in humans where it has highly promising therapeutic potential. Little is known, however, about the structure-function relationships of the different domains of PB. Here, we demonstrate in vitro and in vivo that its C-terminal Cysteine-Rich Domain (CRD) is essential for DNA breakage, joining and transposition and that it binds to specific DNA sequences in the left and right transposon ends, and to an additional unexpectedly internal site at the left end. Using NMR, we show that the CRD adopts the specific fold of the cross-brace zinc finger protein family. We determine the interaction interfaces between the CRD and its target, the 5'-TGCGT-3'/3'-ACGCA-5' motifs found in the left, left internal and right transposon ends, and use NMR results to propose docking models for the complex, which are consistent with our site-directed mutagenesis data. Our results provide support for a model of the PB/DNA interactions in the context of the transpososome, which will be useful for the rational design of PB mutants with increased activity. Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase.,Morellet N, Li X, Wieninger SA, Taylor JL, Bischerour J, Moriau S, Lescop E, Bardiaux B, Mathy N, Assrir N, Betermier M, Nilges M, Hickman AB, Dyda F, Craig NL, Guittet E Nucleic Acids Res. 2018 Jan 27. pii: 4827089. doi: 10.1093/nar/gky044. PMID:29385532[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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